ArticleScientific reports2014
Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Investigating the Effects of Nanogels in Promoting Protein Crystallization.International journal of molecular sciences · 2026Article
- Nucleation of protein crystals in pores and their growth.Biophysical reviews · 2025Review
- A generic cross-seeding approach to protein crystallization.Journal of applied crystallography · 2025Article
- On-the-fly resolution enhancement in X-ray protein crystallography using electric field.European biophysics journal : EBJ · 2025Article
- Heterogeneous Nucleation in Protein Crystallization.Biomimetics (Basel, Switzerland) · 2023Review
- Snake Venom Peptides: Tools of Biodiscovery.Toxins · 2018Review
- Use of Protein Thin Film Organized by External Electric Field as a Template for Protein Crystallization.ACS omega · 2018Article
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Authors and funding
19 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-quality protein crystals of suitable size are an important prerequisite for applying X-ray crystallography to determine the 3-dimensional structure of proteins. However, it is often difficult to obtain protein crystals of appropriate size and quality because nucleation and growth processes can be unsuccessful. Here, we show that by adsorbing proteins onto porous polystyrene-divinylbenzene microspheres (SDB) floating on the surface of the crystallisation solution, a localised high supersaturation region at the surface of the microspheres and a low supersaturation region below the microspheres can coexist in a single solution. The crystals will easily nucleate in the region of high supersaturation, but when they grow to a certain size, they will sediment to the region of low supersaturation and continue to grow. In this way, the probability of crystallisation and crystal quality can be simultaneously increased in a single solution without changing other crystallisation parameters.
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Registered trials
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