ArticleScientific reports2014
In-vivo optical tomography of small scattering specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
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- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- How Non-invasiveFrontiers in physiology · 2020Review
- OptiJ: Open-source optical projection tomography of large organ samples.Scientific reports · 2019Article
- Gaussian Light Model in Brightfield Optical Projection Tomography.Scientific reports · 2019Article
- A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples.BMC biology · 2019Article
- High-resolution ultramicroscopy of the developing and adult nervous system in optically cleared Drosophila melanogaster.Nature communications · 2018Article
- Cousins at work: How combining medical with optical imaging enhances in vivo cell tracking.The international journal of biochemistry & cell biology · 2018Review
- Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM.Light, science & applications · 2018Article
- Optical projection tomography for rapid whole mouse brain imaging.Biomedical optics express · 2017Article
- Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity.Journal of experimental botany · 2017Article
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- Applicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography.Biomedical optics express · 2016Article
- Polarization-sensitive optical projection tomography for muscle fiber imaging.Scientific reports · 2016Article
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6 authors.
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Abstract
Even though in vivo imaging approaches have witnessed several new and important developments, specimens that exhibit high light scattering properties such as Drosophila melanogaster pupae are still not easily accessible with current optical imaging techniques, obtaining images only from subsurface features. This means that in order to obtain 3D volumetric information these specimens need to be studied either after fixation and a chemical clearing process, through an imaging window--thus perturbing physiological development -, or during early stages of development when the scattering contribution is negligible. In this paper we showcase how Optical Projection Tomography may be used to obtain volumetric images of the head eversion process in vivo in Drosophila melanogaster pupae, both in control and headless mutant specimens. Additionally, we demonstrate the use of Helical Optical Projection Tomography (hOPT) as a tool for high throughput 4D-imaging of several specimens simultaneously.
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