ArticleThe European journal of neuroscience2015
Caged compounds for multichromic optical interrogation of neural systems.
Article in The European journal of neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- Geometric principles of dendritic integration of excitation and inhibition in cortical neurons.Science advances · 2026Article
- Review
- CaCell reports · 2024Article
- Bidirectional Neuronal Actuation by Uncaging with Violet and Green Light.Angewandte Chemie (International ed. in English) · 2024Article
- Blue and Green Light Responsive Caged Glutamate.Journal of photochemistry and photobiology. A, Chemistry · 2024Article
- Evaluating spatial and network properties of NMDA-dependent neuronal connectivity in mixed cortical cultures.Brain research · 2022Article
- Strategy for Engineering High Photolysis Efficiency of Photocleavable Protecting Groups through Cation Stabilization.Journal of the American Chemical Society · 2022Article
- Liposome-Tethered Gold Nanoparticles Triggered by Pulsed NIR Light for Rapid Liposome Contents Release and Endosome Escape.Pharmaceutics · 2022Review
- Advances in Two-Photon Imaging in Plants.Plant & cell physiology · 2021Review
- Neural and behavioral control inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Visible-to-NIR-Light Activated Release: From Small Molecules to Nanomaterials.Chemical reviews · 2020Article
- Useful Caged Compounds for Cell Physiology.Accounts of chemical research · 2020Article
- Precise 3D modulation of electro-optical parameters during neurotransmitter uncaging experiments with neurons in vitro.Scientific reports · 2020Article
- Optofluidic control of rodent learning using cloaked caged glutamate.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Near Infrared-Triggered Liposome Cages for Rapid, Localized Small Molecule Delivery.Scientific reports · 2020Article
- Optical control of neuronal ion channels and receptors.Nature reviews. Neuroscience · 2019Review
- Development of photolabile protecting groups and their application to the optochemical control of cell signaling.Current opinion in structural biology · 2019Review
- Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation.Methods in enzymology · 2019Article
- Improved Synthesis of Caged Glutamate and Caging Each Functional Group.ACS chemical neuroscience · 2018Article
- Thermodynamically Stable, Photoreversible Pharmacology in Neurons with One- and Two-Photon Excitation.Angewandte Chemie (International ed. in English) · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Caged compounds are widely used by neurophysiologists to study many aspects of cellular signaling in glia and neurons. Biologically inert before irradiation, they can be loaded into cells via patch pipette or topically applied in situ to a defined concentration; photolysis releases the caged compound in a very rapid and spatially defined way. As caged compounds are exogenous optical probes, they include not only natural products such neurotransmitters, calcium and IP3 but non-natural products such as fluorophores, drugs and antibodies. In this Technical Spotlight we provide a short introduction to the uncaging technique by discussing the nitroaromatic caging chromophores most widely used in such experiments [e.g. α-carboxy-ortho-nitrobenyl (CNB), dimethoxynitrobenzyl (DMNB), 4-methoxy-7-nitroindolinyl (MNI) and 4-carboxymethoxy-7-nitroindolinyl (CDNI)]. We show that recently developed caging chromophores [rutheniumbipyridial (RuBi) and 7-diethylaminocoumarin (DEAC)450] that are photolyzed with blue light (~ 430-480 nm range) can be combined with traditional nitroaromatic caged compounds to enable two-color optical probing of neuronal function. For example, one-photon uncaging of either RuBi-GABA or DEAC450-GABA with a 473-nm laser is facile, and can block nonlinear currents (dendritic spikes or action potentials) evoked by two-photon uncaging of CDNI-Glu at 720 nm. We also show that two-photon uncaging of DEAC450-Glu and CDNI-GABA at 900 and 720 nm, respectively, can be used to fire and block action potentials. Our experiments illustrate that recently developed chromophores have taken uncaging out of the 'monochrome era', in which it has existed since 1978, so as to enable multichromic interrogation of neuronal function with single-synapse precision.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.