ArticlePloS one2014
Devising assisted reproductive technologies for wild-derived strains of mice: 37 strains from five subspecies of Mus musculus.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Highly efficient and low-mosaicismiScience · 2026Article
- Effects of fertilization media on in vitro embryonic development in red-rumped agouti () using a heterologous system with guinea pig oocytes.JBRA assisted reproduction · 2026Article
- Mammalian genome research resources available from the National BioResource Project in Japan.Mammalian genome : official journal of the International Mammalian Genome Society · 2024Review
- Different responses of larval fatty acid profiles to cryopreservation in two commercially important bivalves.Scientific reports · 2024Article
- Inter-subspecies mouse F1 hybrid embryonic stem cell lines newly established for studies of allelic imbalance in gene expression.Experimental animals · 2024Article
- Article
- Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells.Scientific reports · 2023Article
- Efficient genome editing in wild strains of mice using the i-GONAD method.Scientific reports · 2022Article
- Use of anti-inhibin monoclonal antibody for increasing the litter size of mouse strains and its application to in vivo-genome editing technology†.Biology of reproduction · 2022Article
- Mouse in vivo-derived late 2-cell embryos have higher developmental competence after high osmolality vitrification and -80°C preservation than IVF or ICSI embryos.The Journal of reproduction and development · 2022Article
- Mouse resources at the RIKEN BioResource Research Center and the National BioResource Project core facility in Japan.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- Development of assisted reproductive technologies in small animal species for their efficient preservation and production.The Journal of reproduction and development · 2020Review
- Generation of Artificial Gamete and Embryo From Stem Cells in Reproductive Medicine.Frontiers in bioengineering and biotechnology · 2020Review
- Early production of offspring by in vitro fertilization using first-wave spermatozoa from prepubertal male mice.The Journal of reproduction and development · 2019Article
- Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells.Stem cell reports · 2019Article
- Telomere shortening by transgenerational transmission of TNF-α-induced TERRA via ATF7.Nucleic acids research · 2019Article
- CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene.Scientific reports · 2017Article
- Development of reproductive engineering techniques at the RIKEN BioResource Center.Experimental animals · 2017Review
- Successful in vitro fertilization and generation of transgenics in Black and Tan Brachyury (BTBR) mice.Transgenic research · 2016Article
- Article
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Authors and funding
16 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wild-derived mice have long offered invaluable experimental models for mouse genetics because of their high evolutionary divergence from laboratory mice. A number of wild-derived strains are available from the RIKEN BioResource Center (BRC), but they have been maintained as living stocks because of the unavailability of assisted reproductive technology (ART). In this study, we sought to devise ART for 37 wild-derived strains from five subspecies of Mus musculus maintained at the BRC. Superovulation of females was effective (more than 15 oocytes per female) for 34 out of 37 strains by treatment with either equine chorionic gonadotropin or anti-inhibin serum, depending on their genetic background (subspecies). The collected oocytes could be fertilized in vitro at mean rates of 79.0% and 54.6% by the optimized protocol using fresh or frozen-thawed spermatozoa, respectively. They were cryopreserved at the 2-cell stage by vitrification with an ethylene glycol-based solution. In total, 94.6% of cryopreserved embryos survived the vitrification procedure and restored their normal morphology after warming. A conventional embryo transfer protocol could be applied to 25 out of the 35 strains tested. In the remaining 10 strains, live offspring could be obtained by a modified embryo transfer protocol using cyclosporin A treatment and co-transfer of ICR (laboratory mouse strain) embryos. Thus, ART for 37 wild-derived strains was devised successfully and is now routinely used for their preservation and transportation. The information provided here might facilitate broader use and wider distribution of wild-derived mice for biomedical research.
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