ArticlePloS one2014
A fully automated microfluidic femtosecond laser axotomy platform for nerve regeneration studies in C. elegans.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Impairment of neuronal activity occurs at the early stages of the aggregation cascade of Aβ1-42 and mutant Tau.Disease models & mechanisms · 2026Article
- SMORES: a simple microfluidic operating room for the examination and surgery of Stentor coeruleus.Scientific reports · 2024Article
- Worm-Based Diagnosis Combining Microfluidics toward Early Cancer Screening.Micromachines · 2024Review
- SMORES: A Simple Microfluidic Operating Room for the Examination and Surgery ofbioRxiv : the preprint server for biology · 2024Article
- Difference in Intestine Content ofMicromachines · 2023Article
- Spatial single-cell sequencing of meiosis I arrested oocytes indicates acquisition of maternal transcripts from the soma.Cell reports · 2023Article
- Article
- High-resolution imaging and analysis of the intestinal bacterial load ofRSC advances · 2023Article
- Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing.Nature methods · 2023Article
- Microfluidic approaches forAnimal cells and systems · 2020Review
- Versatile cell ablation tools and their applications to study loss of cell functions.Cellular and molecular life sciences : CMLS · 2019Review
- Microfluidic Technologies for High Throughput Screening Through Sorting and On-Chip Culture ofMolecules (Basel, Switzerland) · 2019Review
- An automated microfluidic device for time-lapse imaging of mouse embryonic stem cells.Biomicrofluidics · 2019Article
- Advances in ex vivo models and lab-on-a-chip devices for neural tissue engineering.Biomaterials · 2019Review
- Surface acoustic waves enable rotational manipulation of Caenorhabditis elegans.Lab on a chip · 2019Article
- High-Content Microfluidic Screening Platform Used To Identify σ2R/Tmem97 Binding Ligands that Reduce Age-Dependent Neurodegeneration in C. elegans SC_APP Model.ACS chemical neuroscience · 2018Article
- Article
- A multi-trap microfluidic chip enabling longitudinal studies of nerve regeneration in Caenorhabditis elegans.Scientific reports · 2017Article
- High-throughput screening in the C. elegans nervous system.Molecular and cellular neurosciences · 2017Review
- Axon regeneration in C. elegans: Worming our way to mechanisms of axon regeneration.Experimental neurology · 2017Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Femtosecond laser nanosurgery has been widely accepted as an axonal injury model, enabling nerve regeneration studies in the small model organism, Caenorhabditis elegans. To overcome the time limitations of manual worm handling techniques, automation and new immobilization technologies must be adopted to improve throughput in these studies. While new microfluidic immobilization techniques have been developed that promise to reduce the time required for axotomies, there is a need for automated procedures to minimize the required amount of human intervention and accelerate the axotomy processes crucial for high-throughput. Here, we report a fully automated microfluidic platform for performing laser axotomies of fluorescently tagged neurons in living Caenorhabditis elegans. The presented automation process reduces the time required to perform axotomies within individual worms to ∼17 s/worm, at least one order of magnitude faster than manual approaches. The full automation is achieved with a unique chip design and an operation sequence that is fully computer controlled and synchronized with efficient and accurate image processing algorithms. The microfluidic device includes a T-shaped architecture and three-dimensional microfluidic interconnects to serially transport, position, and immobilize worms. The image processing algorithms can identify and precisely position axons targeted for ablation. There were no statistically significant differences observed in reconnection probabilities between axotomies carried out with the automated system and those performed manually with anesthetics. The overall success rate of automated axotomies was 67.4±3.2% of the cases (236/350) at an average processing rate of 17.0±2.4 s. This fully automated platform establishes a promising methodology for prospective genome-wide screening of nerve regeneration in C. elegans in a truly high-throughput manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.