Evidence map›Paper›PMID 25470061›Full record

ArticleNature2015

Structure of a mammalian ryanodine receptor.

Ran Zalk, Oliver B Clarke, Amédée des Georges, Robert A Grassucci, Steven Reiken, Filippo Mancia, Wayne A Hendrickson, Joachim Frank, Andrew R Marks

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 239 papers.

0numbers the graph read from it
0cells of the map it votes in
239citing papers in PubMed
22.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

239 citing papers in PubMed, 420 citations in OpenAlex.

  1. BioMed research international · 2019
    Trial
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Balancing of immune activation and suppression during phage infection.bioRxiv : the preprint server for biology · 2026
    Article
  8. Cylindrical Crystallization of CaInternational journal of molecular sciences · 2026
    Review
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Effects of aging on calcium channels in skeletal muscle.Frontiers in molecular biosciences · 2025
    Review

179 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Ran ZalkDepartment of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032, USA.
Oliver B ClarkeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
Amédée des GeorgesDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
Robert A Grassucci1] Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA [2] Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA.
Steven ReikenDepartment of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032, USA.
Filippo ManciaDepartment of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032, USA.
Wayne A Hendrickson1] Department of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032, USA [2] Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
Joachim Frank1] Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA [2] Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA [3] Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Andrew R Marks1] Department of Physiology and Cellular Biophysics, Columbia University, New York, New York 10032, USA [2] Department of Medicine, Columbia University, New York, New York 10032, USA [3] Wu Center for Molecular Cardiology, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
Columbia University · US

Funding

Structural Genomics on Membrane ProteinsU54GM095315 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI ROST, BURKHARD · 2010 to 2014
$15.2M
REGULATION OF RYANODINE RECEPTORS BY ALLOSTERIC MODULATIONP01HL081172 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI KARLIN, ARTHUR · 2007 to 2011
$7.3M
MOLECULAR BASIS OF HEART FAILURER01HL061503 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MARKS, ANDREW ROBERT · 1998 to 2016
$6.5M
STRUCTURE ANALYSIS OF MACROMOLECULAR ASSEMBLIESR01GM029169 · NIGMS · WADSWORTH CENTER · PI FRANK, JOACHIM · 1985 to 2019
$6.1M
Intracellular calcium leak and muscle functionR01AR060037 · NIAMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MARKS, ANDREW ROBERT · 2010 to 2014
$1.7M
Regulation of cardiac ion channel function via allosteric modulatorsR01HL083418 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MARKS, ANDREW ROBERT · 2006 to 2007
$841k
Howard Hughes Medical InstituteNHLBI NIH HHS P01 HL081172NHLBI NIH HHS R01 HL061503NHLBI NIH HHS R01HL061503NHLBI NIH HHS R01 HL083418NIAMS NIH HHS R01 AR060037NIAMS NIH HHS R01AR060037NIGMS NIH HHS R01 GM029169NIGMS NIH HHS R01GM29169NIGMS NIH HHS U54 GM095315NIGMS NIH HHS U54GM095315
6 · The paper itself

Abstract

Ryanodine receptors (RyRs) mediate the rapid release of calcium (Ca(2+)) from intracellular stores into the cytosol, which is essential for numerous cellular functions including excitation-contraction coupling in muscle. Lack of sufficient structural detail has impeded understanding of RyR gating and regulation. Here we report the closed-state structure of the 2.3-megadalton complex of the rabbit skeletal muscle type 1 RyR (RyR1), solved by single-particle electron cryomicroscopy at an overall resolution of 4.8 Å. We fitted a polyalanine-level model to all 3,757 ordered residues in each protomer, defining the transmembrane pore in unprecedented detail and placing all cytosolic domains as tertiary folds. The cytosolic assembly is built on an extended α-solenoid scaffold connecting key regulatory domains to the pore. The RyR1 pore architecture places it in the six-transmembrane ion channel superfamily. A unique domain inserted between the second and third transmembrane helices interacts intimately with paired EF-hands originating from the α-solenoid scaffold, suggesting a mechanism for channel gating by Ca(2+).

Indexed as

AnimalsCalciumCell MembraneCryoelectron MicroscopyCytosolIon Channel GatingMuscle, SkeletalProtein Structure, TertiaryRabbitsRyanodine Receptor Calcium Release ChannelTacrolimus Binding ProteinsCalciumRyanodine Receptor Calcium Release ChannelTacrolimus Binding Proteins

Identifiers

PMID25470061
PMCPMC4300236
OpenAlexW2021630348

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.