ArticleSmall (Weinheim an der Bergstrasse, Germany)2015
Magnetic tweezers-based 3D microchannel electroporation for high-throughput gene transfection in living cells.
Article in Small (Weinheim an der Bergstrasse, Germany), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 91 citations in OpenAlex.
- Microfluidics for cell therapy and manufacturing in oncology and regenerative medicine.Lab on a chip · 2026Review
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- In Situ Electroporation on PERFECT Filter for High-Efficiency and High-Viability Tumor Cell Labeling.Micromachines · 2022Article
- In Situ Deployment of Engineered Extracellular Vesicles into the Tumor Niche via Myeloid-Derived Suppressor Cells.Advanced healthcare materials · 2022Article
- An equivalent circuit model for localized electroporation on porous substrates.Biosensors & bioelectronics · 2022Article
- Manipulation of single cells via a Stereo Acoustic Streaming Tunnel (SteAST).Microsystems & nanoengineering · 2022Article
- Fabrication and use of silicon hollow-needle arrays to achieve tissue nanotransfection in mouse tissue in vivo.Nature protocols · 2021Review
- Microfluidic and Nanofluidic Intracellular Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Massively-Parallelized, Deterministic Mechanoporation for Intracellular Delivery.Nano letters · 2020Article
- Active particles as mobile microelectrodes for selective bacteria electroporation and transport.Science advances · 2020Article
- On-chip multiplexed single-cell patterning and controllable intracellular delivery.Microsystems & nanoengineering · 2020Article
- Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells.Scientific reports · 2019Article
- A Review on Electroporation-Based Intracellular Delivery.Molecules (Basel, Switzerland) · 2018Review
- Microfluidic-Based Single-Cell Study: Current Status and Future Perspective.Molecules (Basel, Switzerland) · 2018Review
- Ionomycin-Induced Changes in Membrane Potential Alter Electroporation Outcomes in HL-60 Cells.Biophysical journal · 2018Article
- Investigation of ac-magnetic field stimulated nanoelectroporation of magneto-electric nano-drug-carrier inside CNS cells.Scientific reports · 2017Article
- Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture.Biomicrofluidics · 2017Review
- Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Separation by nanoparticles plasmonic resonance with low stress in microfluidics channel (analytical and design).IET nanobiotechnology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
A novel high-throughput magnetic tweezers-based 3D microchannel electroporation system capable of transfecting 40 000 cells/cm(2) on a single chip for gene therapy, regenerative medicine, and intracellular detection of target mRNA for screening cellular heterogeneity is reported. A single cell or an ordered array of individual cells are remotely guided by programmable magnetic fields to poration sites with high (>90%) cell alignment efficiency to enable various transfection reagents to be delivered simultaneously into the cells. The present technique, in contrast to the conventional vacuum-based approach, is significantly gentler on the cellular membrane yielding >90% cell viability and, moreover, allows transfected cells to be transported for further analysis. Illustrating the versatility of the system, the GATA2 molecular beacon is delivered into leukemia cells to detect the regulation level of the GATA2 gene that is associated with the initiation of leukemia. The uniform delivery and a sharp contrast of fluorescence intensity between GATA2 positive and negative cells demonstrate key aspects of the platform for gene transfer, screening and detection of targeted intracellular markers in living cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.