Evidence map›Paper›PMID 25469509›Full record

ArticlePloS one2014

Regionally distinct alterations in the composition of the gut microbiota in rats with streptozotocin-induced diabetes.

Roland Wirth, Nikolett Bódi, Gergely Maróti, Mária Bagyánszki, Petra Talapka, Éva Fekete, Zoltán Bagi, Kornél L Kovács

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.

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  18. Gut-Pancreas-Liver Axis as a Target for Treatment of NAFLD/NASH.International journal of molecular sciences · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Roland WirthDepartment of Biotechnology, University of Szeged, Szeged, Hungary.
Nikolett BódiDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary.
Gergely MarótiInstitute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
Mária BagyánszkiDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary.
Petra TalapkaDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary.
Éva FeketeDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary.
Zoltán BagiDepartment of Biotechnology, University of Szeged, Szeged, Hungary.
Kornél L KovácsDepartment of Biotechnology, University of Szeged, Szeged, Hungary; Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary; Department of Oral Biology and Experimental Dentistry, University of Szeged, Szeged, Hungary.
University of Szeged · HUHungarian Academy of Sciences · HU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to map the microbiota distribution along the gut and establish whether colon/faecal samples from diabetic rats adequately reflect the diabetic alterations in the microbiome. Streptozotocin-treated rats were used to model type 1 diabetes mellitus (T1D). Segments of the duodenum, ileum and colon were dissected, and the microbiome of the lumen material was analysed by using next-generation DNA sequencing, from phylum to genus level. The intestinal luminal contents were compared between diabetic, insulin-treated diabetic and healthy control rats. No significant differences in bacterial composition were found in the luminal contents from the duodenum of the experimental animal groups, whereas distinct patterns were seen in the ileum and colon, depending on the history of the luminal samples. Ileal samples from diabetic rats exhibited particularly striking alterations, while the richness and diversity obscured some of the modifications in the colon. Characteristic rearrangements in microbiome composition and diversity were detected after insulin treatment, though the normal gut flora was not restored. The Proteobacteria displayed more pronounced shifts than those of the predominant phyla (Firmicutes and Bacteroidetes) in the rat model of T1D. Diabetes and insulin replacement affect the composition of the gut microbiota in different, gut region-specific manners. The luminal samples from the ileum appear more suitable for diagnostic purposes than the colon/faeces. The Proteobacteria should be at the focus of diagnosis and potential therapy. Klebsiella are recommended as biomarkers of T1D.

Indexed as

AnimalsBacteriaColonDiabetes Mellitus, ExperimentalDNA, BacterialFecesHigh-Throughput Nucleotide SequencingIleumKlebsiellaMaleMicrobiotaProteobacteriaRatsRats, WistarSequence Analysis, DNAStreptozocinDNA, BacterialStreptozocin

Identifiers

PMID25469509
PMCPMC4254516
OpenAlexW2158214685

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.