ArticleBioResearch open access2014
An alternative approach to decellularize whole porcine heart.
Article in BioResearch open access, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Biological tissue engineering for Fontan failure using decellularized extracellular matrix: a systematic review.Journal of translational medicine · 2026Pooled it
- Decellularized cardiac ECM: A tissue-specific bioactive platform for drug release and cell delivery.Journal of controlled release : official journal of the Controlled Release Society · 2026Review
- Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices.Bioengineering (Basel, Switzerland) · 2025Review
- A sterilization method for human decellularized vaginal matrices.Scientific reports · 2024Article
- Cells and Materials for Cardiac Repair and Regeneration.Journal of clinical medicine · 2023Review
- Functional acellular matrix for tissue repair.Materials today. Bio · 2023Review
- Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges.International journal of molecular sciences · 2022Review
- Modeling the human heart ex vivo-current possibilities and strive for future applications.Journal of tissue engineering and regenerative medicine · 2022Review
- ECM roles and biomechanics in cardiac tissue decellularization.American journal of physiology. Heart and circulatory physiology · 2022Review
- Decellularization of Full Heart-Optimizing the Classical Sodium-Dodecyl-Sulfate-Based Decellularization Protocol.Bioengineering (Basel, Switzerland) · 2022Article
- Optimization of Complete Rat Heart Decellularization Using Artificial Neural Networks.Micromachines · 2022Article
- Decellularization Strategies for Regenerating Cardiac and Skeletal Muscle Tissues.Frontiers in bioengineering and biotechnology · 2022Review
- Applications of Tissue Decellularization Techniques in Ventricular Myocardial Biofabrication.Frontiers in bioengineering and biotechnology · 2022Review
- Therapeutic Acellular Scaffolds for Limiting Left Ventricular Remodelling-Current Status and Future Directions.International journal of molecular sciences · 2021Review
- In vitro comparison of harvesting site effects on cardiac extracellular matrix hydrogels.Journal of biomedical materials research. Part A · 2021Article
- An exploratory assessment of stretch-induced transmural myocardial fiber kinematics in right ventricular pressure overload.Scientific reports · 2021Article
- Article
- Young at Heart: Combining Strategies to Rejuvenate Endogenous Mechanisms of Cardiac Repair.Frontiers in bioengineering and biotechnology · 2020Review
- Cardiac tissue-derived extracellular matrix scaffolds for myocardial repair: advantages and challenges.Regenerative biomaterials · 2019Review
- Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration.Advanced healthcare materials · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scaffold characteristics are decisive for repopulating the acellular tissue with cells. A method to produce such a scaffold from intact organ requires a customized decellularization protocol. Here, we have decellularized whole, intact porcine hearts by serial perfusion and agitation of hypotonic solution, an ionic detergent (4% sodium deoxycholate), and a nonionic detergent (1% Triton X-100). The resultant matrix was characterized for its degree of decellularization, morphological and functional integrity. The protocol used resulted in extensive decellularization of the cardiac tissue, but the cytoskeletal elements (contractile apparatus) of cardiomyocytes remained largely unaffected by the procedure although their membranous organelles were completely absent. Further, several residual angiogenic growth factors were found to be present in the decellularized tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.