ArticleBiomedical reports2015
A novel method for co-culture with Müller cells and microglia in rat retina
Article in Biomedical reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Store-operated calcium entry drives alcohol-exacerbated neuroinflammation in retinal degeneration.Cell death discovery · 2026Article
- NDR2 Kinase Regulates Microglial Metabolic Adaptation and Inflammatory Response: Critical Role in Glucose-Dependent Functional Plasticity.International journal of molecular sciences · 2025Article
- Exosomal MiRNA Transfer between Retinal Microglia and RPE.International journal of molecular sciences · 2020Article
- Blockade of microglial adenosine AGlia · 2019Article
- Immune Mediated Degeneration and Possible Protection in Glaucoma.Frontiers in neuroscience · 2019Review
- Viral Retinopathy in Experimental Models of Zika Infection.Investigative ophthalmology & visual science · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microglia and Müller cells are glial cells of the retina and constitute a functional link between neurons and vessels. The aim of the present study was to introduce a novel method of co-culture with Müller cells and microglia in rat retina. A camera was used to analyze all the cell changes. Immunofluorescence staining of glutamine synthetase and OX-42 were used for the identification of Müller cells and microglial, respectively. On day 1, all the cell types from the retina were round or oval and floating in the medium. On the following days, microglial cells were adherent and proliferated. Müller cells stretched and quickly proliferated. On days 12-15, microglial cells were floating in the medium. Following agitation, microglial cells became quickly detached from the flask walls, whereas Müller cells remained adherent. In conclusion, agitation is an effective way to separate microglial cells from Müller cells. The time of detachment and the speed of agitation are essential. Co-culture with Müller cells and microglia in the retina is economical and useful for future methods in microglia and Müller cell research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.