ArticleProceedings of the National Academy of Sciences of the United States of America2014
Type IV pili interactions promote intercellular association and moderate swarming of Pseudomonas aeruginosa.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 86 citations in OpenAlex.
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- Generative active learning guided discovery of a tobramycin and ciprofloxacin adjuvant against multidrug-resistantActa pharmaceutica Sinica. B · 2026Article
- ZnO Nanocrystals InhibitInternational journal of nanomedicine · 2026Article
- Biofilm and Outer Membrane Vesicle Formation in ESKAPE Gram-Negative Bacteria: A Comprehensive Review.International journal of molecular sciences · 2025Review
- Flagellum-driven motility enhancesApplied and environmental microbiology · 2025Article
- The role of PilU in the surface behaviors ofmLife · 2025Article
- Article
- PilY1 regulates the dynamic architecture of the type IV pilus machine in Pseudomonas aeruginosa.Nature communications · 2024Article
- Multiple pathways impact the swarming motility ofMicrobiology spectrum · 2024Article
- HowmBio · 2024Article
- Spatial transcriptome-guided multi-scale framework connects P. aeruginosa metabolic states to oxidative stress biofilm microenvironment.PLoS computational biology · 2024Article
- Multiple Pathways Impact Swarming Motility ofbioRxiv : the preprint server for biology · 2024Article
- Biofilm formation: mechanistic insights and therapeutic targets.Molecular biomedicine · 2023Review
- The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks inMicroorganisms · 2023Article
- Swarming ofBiophysics reviews · 2023Review
- Effectiveness of Pseudomonas aeruginosa type VI secretion system relies on toxin potency and type IV pili-dependent interaction.PLoS pathogens · 2023Article
- AFM Force Mapping Elucidates Pilus Deployment and Key Lifestyle-Dependent Surface Properties inLangmuir : the ACS journal of surfaces and colloids · 2023Article
- Effect of Micro-Patterned Mucin on Quinolone and Rhamnolipid Profiles of MucoidACS infectious diseases · 2023Article
- Mixed-species bacterial swarms show an interplay of mixing and segregation across scales.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Pseudomonas aeruginosa is a ubiquitous bacterium that survives in many environments, including as an acute and chronic pathogen in humans. Substantial evidence shows that P. aeruginosa behavior is affected by its motility, and appendages known as flagella and type IV pili (TFP) are known to confer such motility. The role these appendages play when not facilitating motility or attachment, however, is unclear. Here we discern a passive intercellular role of TFP during flagellar-mediated swarming of P. aeruginosa that does not require TFP extension or retraction. We studied swarming at the cellular level using a combination of laboratory experiments and computational simulations to explain the resultant patterns of cells imaged from in vitro swarms. Namely, we used a computational model to simulate swarming and to probe for individual cell behavior that cannot currently be otherwise measured. Our simulations showed that TFP of swarming P. aeruginosa should be distributed all over the cell and that TFP-TFP interactions between cells should be a dominant mechanism that promotes cell-cell interaction, limits lone cell movement, and slows swarm expansion. This predicted physical mechanism involving TFP was confirmed in vitro using pairwise mixtures of strains with and without TFP where cells without TFP separate from cells with TFP. While TFP slow swarm expansion, we show in vitro that TFP help alter collective motion to avoid toxic compounds such as the antibiotic carbenicillin. Thus, TFP physically affect P. aeruginosa swarming by actively promoting cell-cell association and directional collective motion within motile groups to aid their survival.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.