ArticleProceedings of the National Academy of Sciences of the United States of America2014
Observation of ice-like water layers at an aqueous protein surface.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 174 citations in OpenAlex.
- An Ultrapotent, Ultraeconomical, Antifreeze Polypeptide.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Residue-Specific Structural and Dynamical Coupling of Protein and Hydration Water Revealed by Molecular Dynamics Simulations.Biomolecules · 2025Article
- Interaction of Blood and Bacteria with Slippery Hydrophilic Surfaces.Advanced materials interfaces · 2024Article
- True Origin of Amide I Shifts Observed in Protein Spectra Obtained with Sum Frequency Generation Spectroscopy.The journal of physical chemistry letters · 2023Article
- Poly(vinyl alcohol) Molecular Bottlebrushes Nucleate Ice.Biomacromolecules · 2022Article
- Electron microscopy and calorimetry of proteins in supercooled water.Scientific reports · 2022Article
- Ice Recrystallization Inhibition Is Insufficient to Explain Cryopreservation Abilities of Antifreeze Proteins.Biomacromolecules · 2022Article
- What can S-shaped potential profiles tell us about the mechanism of membrane potential generation?European biophysics journal : EBJ · 2021Article
- Disaccharide Residues are Required for Native Antifreeze Glycoprotein Activity.Biomacromolecules · 2021Article
- Specific Ion-Protein Interactions Influence Bacterial Ice Nucleation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Characterization of microbial antifreeze protein with intermediate activity suggests that a bound-water network is essential for hyperactivity.Scientific reports · 2021Article
- Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Ice-nucleating proteins are activated by low temperatures to control the structure of interfacial water.Nature communications · 2021Article
- An introduction to cryo-FIB-SEM cross-sectioning of frozen, hydrated Life Science samples.Journal of microscopy · 2021Article
- Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.Biomolecules · 2020Review
- Peptidic Antifreeze Materials: Prospects and Challenges.International journal of molecular sciences · 2019Review
- Site-specific conjugation of antifreeze proteins onto polymer-stabilized nanoparticles.Polymer chemistry · 2019Article
- Ice recrystallization is strongly inhibited when antifreeze proteins bind to multiple ice planes.Scientific reports · 2019Article
- Collective behavior of oscillating electric dipoles.Scientific reports · 2018Article
- Antifreeze protein hydration waters: Unstructured unless bound to ice.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We study the properties of water at the surface of an antifreeze protein with femtosecond surface sum frequency generation spectroscopy. We find clear evidence for the presence of ice-like water layers at the ice-binding site of the protein in aqueous solution at temperatures above the freezing point. Decreasing the temperature to the biological working temperature of the protein (0 °C to -2 °C) increases the amount of ice-like water, while a single point mutation in the ice-binding site is observed to completely disrupt the ice-like character and to eliminate antifreeze activity. Our observations indicate that not the protein itself but ordered ice-like water layers are responsible for the recognition and binding to ice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.