Evidence map›Paper›PMID 25468666›Full record

ArticleJournal of biomechanics2015

Dynamic nanomechanics of individual bone marrow stromal cells and cell-matrix composites during chondrogenic differentiation.

BoBae Lee, Lin Han, Eliot H Frank, Alan J Grodzinsky, Christine Ortiz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomechanics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Aggrecan: Approaches to Study Biophysical and Biomechanical Properties.Methods in molecular biology (Clifton, N.J.) · 2022
    Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Osteoarthritis year in review 2015: mechanics.Osteoarthritis and cartilage · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

BoBae LeeDepartment of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Lin HanSchool of Biomedical Engineering, Science and Health Systems, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA.
Eliot H FrankCenter for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Alan J GrodzinskyCenter for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address: alg@mit.edu.
Christine OrtizDepartment of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. Electronic address: cortiz@mit.edu.

Funding

Cartilage Repair Using Self Assembling Peptide ScaffoldsR01AR060331 · NIAMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GRODZINSKY, ALAN J. · 2012 to 2016
$1.7M
ELECTROMECHANICS OF CARTILAGE: SYNTHESIS &DEGRADATIIONR01AR033236 · NIAMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GRODZINSKY, ALAN J. · 1986 to 2010
$1.5M
CARTILAGE METABOLIC RESPOSE TO LOADING: TARGET MOLECULESR37AR033236 · NIAMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GRODZINSKY, ALAN J · 1995 to 2004
$1.2M
NIAMS NIH HHS AR060331NIAMS NIH HHS R01 AR033236NIAMS NIH HHS R01 AR060331NIAMS NIH HHS R37 AR033236
6 · The paper itself

Abstract

Dynamic nanomechanical properties of bovine bone marrow stromal cells (BMSCs) and their newly synthesized cartilage-like matrices were studied at nanometer scale deformation amplitudes. The increase in their dynamic modulus, |E(*)| (e.g., 2.4±0.4 kPa at 1 Hz to 9.7±0.2 kPa at 316 Hz at day 21, mean±SEM), and phase angle, δ, (e.g., 15±2° at 1 Hz to 74±1° at 316 Hz at day 21) with increasing frequency were attributed to the fluid flow induced poroelasticity, governed by both the newly synthesized matrix and the intracellular structures. The absence of culture duration dependence suggested that chondrogenesis of BMSCs had not yet resulted in the formation of a well-organized matrix with a hierarchical structure similar to cartilage. BMSC-matrix composites demonstrated different poro-viscoelastic frequency-dependent mechanical behavior and energy dissipation compared to chondrocyte-matrix composites due to differences in matrix molecular constituents, structure and cell properties. This study provides important insights into the design of optimal protocols for tissue-engineered cartilage products using chondrocytes and BMSCs.

Indexed as

Cell DifferentiationChondrogenesisAnimalsBiomechanical PhenomenaCartilageCattleCells, CulturedChondrocytesExtracellular MatrixMesenchymal Stem CellsMicroscopy, Atomic ForceTissue EngineeringBone marrow stromal cellsCartilageNanomechanicsPoroelasticityTissue-engineering

Identifiers

PMID25468666
PMCPMC6713483

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.