ArticleBiophysical journal2014
Cytoplasmic dynamics reveals two modes of nucleoid-dependent mobility.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 52 citations in OpenAlex.
- OmniSegger: A time-lapse image analysis pipeline for bacterial cells.PLoS computational biology · 2025Article
- Manipulating subcellular protein localization to enhance target protein accumulation in minicells.Journal of biological engineering · 2025Article
- Modeling Diffusion Between Regions with Different Diffusion Coefficients.IEEE transactions on molecular, biological, and multi-scale communications · 2024Article
- ExTrack characterizes transition kinetics and diffusion in noisy single-particle tracks.The Journal of cell biology · 2023Article
- Dynamics of Proteins and Macromolecular Machines in Escherichia coli.EcoSal Plus · 2021Review
- Article
- Glass-like characteristics of intracellular motion in human cells.Biophysical journal · 2021Article
- Transient non-specific DNA binding dominates the target search of bacterial DNA-binding proteins.Molecular cell · 2021Article
- Fluctuating Diffusivity of RNA-Protein Particles: Analogy with Thermodynamics.Entropy (Basel, Switzerland) · 2021Article
- Superstatistical modelling of protein diffusion dynamics in bacteria.Journal of the Royal Society, Interface · 2021Article
- Article
- Nucleoid-mediated positioning and transport in bacteria.Current genetics · 2020Review
- Effects of soft interactions and bound mobility on diffusion in crowded environments: a model of sticky and slippery obstacles.Physical biology · 2017Article
- Probing bacterial cell biology using image cytometry.Molecular microbiology · 2017Article
- Cytoplasmic RNA-Protein Particles Exhibit Non-Gaussian Subdiffusive Behavior.Biophysical journal · 2017Article
- Spatial organization of bacterial transcription and translation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Bacterial partition complexes segregate within the volume of the nucleoid.Nature communications · 2016Article
- Escherichia coli Chromosomal Loci Segregate from Midcell with Universal Dynamics.Biophysical journal · 2016Article
- Physical Modeling of Dynamic Coupling between Chromosomal Loci.Biophysical journal · 2016Article
- High-throughput cell-cycle imaging opens new doors for discovery.Current genetics · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been proposed that forces resulting from the physical exclusion of macromolecules from the bacterial nucleoid play a central role in organizing the bacterial cell, yet this proposal has not been quantitatively tested. To investigate this hypothesis, we mapped the generic motion of large protein complexes in the bacterial cytoplasm through quantitative analysis of thousands of complete cell-cycle trajectories of fluorescently tagged ectopic MS2-mRNA complexes. We find the motion of these complexes in the cytoplasm is strongly dependent on their spatial position along the long axis of the cell, and that their dynamics are consistent with a quantitative model that requires only nucleoid exclusion and membrane confinement. This analysis also reveals that the nucleoid increases the mobility of MS2-mRNA complexes, resulting in a fourfold increase in diffusion coefficients between regions of the lowest and highest nucleoid density. These data provide strong quantitative support for two modes of nucleoid action: the widely accepted mechanism of nucleoid exclusion in organizing the cell and a newly proposed mode, in which the nucleoid facilitates rapid motion throughout the cytoplasm.
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