ArticleBiophysical journal2014
Scanning x-ray nanodiffraction on Dictyostelium discoideum.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 36 citations in OpenAlex.
- Fast-scanning small-angle X-ray scattering of hydrated biological cells.Journal of synchrotron radiation · 2026Article
- Sarcomere, troponin, and myosin X-ray diffraction signals can be resolved in single cardiomyocytes.Biophysical journal · 2024Article
- Building a Cell House from Cellulose: The Case of the Soil AcidobacteriumMicroorganisms · 2022Article
- X-Ray Structural Analysis of Single Adult Cardiomyocytes: Tomographic Imaging and Microdiffraction.Biophysical journal · 2020Article
- Large field-of-view scanning small-angle X-ray scattering of mammalian cells.Journal of synchrotron radiation · 2020Article
- The 2018 correlative microscopy techniques roadmap.Journal of physics D: Applied physics · 2018Review
- Correlative microscopy approach for biology using X-ray holography, X-ray scanning diffraction and STED microscopy.Nature communications · 2018Article
- Actin bundles cross-linked with α-actinin studied by nanobeam X-ray diffraction.European biophysics journal : EBJ · 2016Article
- X-Ray Micro- and Nanodiffraction Imaging on Human Mesenchymal Stem Cells and Differentiated Cells.Biophysical journal · 2016Article
- Cellular diffraction: scanning x-ray nanodiffraction from living cells.Biophysical journal · 2014Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have performed scanning x-ray nanobeam diffraction experiments on single cells of the amoeba Dictyostelium discoideum. Cells have been investigated in 1), freeze-dried, 2), frozen-hydrated (vitrified), and 3), initially alive states. The spatially resolved small-angle x-ray scattering signal shows characteristic streaklike patterns in reciprocal space, which we attribute to fiber bundles of the actomyosin network. From the intensity distributions, an anisotropy parameter can be derived that indicates pronounced local variations within the cell. In addition to nanobeam small-angle x-ray scattering, we have evaluated the x-ray differential phase contrast in view of the projected electron density. Different experimental aspects of the x-ray experiment, sample preparation, and data analysis are discussed. Finally, the x-ray results are correlated with optical microscopy (differential phase contrast and confocal microscopy of mutant strains with fluorescently labeled actin and myosin II), which have been carried out in live and fixed states, including optical microscopy under cryogenic conditions.
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