ArticleBiophysical journal2014
Spatially defined EGF receptor activation reveals an F-actin-dependent phospho-Erk signaling complex.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- A comparative analysis of paxillin and Hic-5 proximity interactomes.Cytoskeleton (Hoboken, N.J.) · 2025Article
- From Crypts to Cancer: A Holistic Perspective on Colorectal Carcinogenesis and Therapeutic Strategies.International journal of molecular sciences · 2024Review
- Surface-Patterned DNA Origami Rulers Reveal Nanoscale Distance Dependency of the Epidermal Growth Factor Receptor Activation.Nano letters · 2024Article
- Cytoplasmic p53 aggregates accumulated in p53-mutated cancer correlate with poor prognosis.PNAS nexus · 2022Article
- Unraveling the molecular mechanisms of hyperlipidemia using integrated lncRNA and mRNA microarray data.Experimental and therapeutic medicine · 2022Article
- Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Subcellular Dynamic Immunopatterning of Cytosolic Protein Complexes on Microstructured Polymer Substrates.ACS sensors · 2021Article
- EGFRvIII uses intrinsic and extrinsic mechanisms to reduce glioma adhesion and increase migration.Journal of cell science · 2020Article
- Article
- Tunable DNA Hybridization Enables Spatially and Temporally Controlled Surface-Anchoring of Biomolecular Cargo.Langmuir : the ACS journal of surfaces and colloids · 2018Article
- Ligand Density and Nanoparticle Clustering Cooperate in the Multivalent Amplification of Epidermal Growth Factor Receptor Activation.ACS nano · 2018Article
- Paxillin actions in the nucleus.Steroids · 2018Review
- Mechanisms of epidermal growth factor receptor signaling as characterized by patterned ligand activation and mutational analysis.Biochimica et biophysica acta. Biomembranes · 2017Review
- The FcεRI Signaling Cascade and Integrin Trafficking Converge at Patterned Ligand Surfaces.Molecular biology of the cell · 2017Article
- Inhibition of Receptor Dimerization as a Novel Negative Feedback Mechanism of EGFR Signaling.PloS one · 2015Article
- Pinning down the EGF receptor.Biophysical journal · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We investigated the association of signaling proteins with epidermal growth factor (EGF) receptors (EGFR) using biotinylated EGF bound to streptavidin that is covalently coupled in an ordered array of micron-sized features on silicon surfaces. Using NIH-3T3 cells stably expressing EGFR, we observe concentration of fluorescently labeled receptors and stimulated tyrosine phosphorylation that are spatially confined to the regions of immobilized EGF and quantified by cross-correlation analysis. We observe recruitment of phosphorylated paxillin to activated EGFR at these patterned features, as well as β1-containing integrins that preferentially localize to more peripheral EGF features, as quantified by radial fluorescence analysis. In addition, we detect recruitment of EGFP-Ras, MEK, and phosphorylated Erk to patterned EGF in a process that depends on F-actin and phosphoinositides. These studies reveal and quantify the coformation of multiprotein EGFR signaling complexes at the plasma membrane in response to micropatterned growth factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.