ArticleBiophysical journal2014
Ligand-mediated friction determines morphodynamics of spreading T cells.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Celldetective, an AI-enhanced image analysis tool for unraveling dynamic cell interactions.eLife · 2026Article
- Inference of the 3D pressure field exerted by a single cell from a thin membrane transverse deformation.The European physical journal. E, Soft matter · 2026Article
- Quantitative Microscopy for Cell-Surface and Cell-Cell Interactions in Immunology.Bio-protocol · 2025Article
- Antigen mobility regulates the dynamics and precision of antigen capture in the B cell immune synapse.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanical control of antigen detection and discrimination by T and B cell receptors.Biophysical journal · 2024Review
- Morphodynamics of T-lymphocytes: Scanning to spreading.Biophysical journal · 2024Review
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- Mechanical forces amplify TCR mechanotransduction in T cell activation and function.Applied physics reviews · 2024Review
- Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction.Scientific reports · 2023Article
- Mechanosurveillance: Tiptoeing T Cells.Frontiers in immunology · 2022Review
- Molecular Biomechanics Controls Protein Mixing and Segregation in Adherent Membranes.International journal of molecular sciences · 2021Article
- Controlling T cells spreading, mechanics and activation by micropatterning.Scientific reports · 2021Article
- Colorectal cysts as a validating tool for CAR therapy.BMC biotechnology · 2020Article
- Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.Frontiers in immunology · 2020Article
- Membrane Organization and Physical Regulation of Lymphocyte Antigen Receptors: A Biophysicist's Perspective.The Journal of membrane biology · 2019Review
- Extracellular Matrix Geometry and Initial Adhesive Position Determine Stress Fiber Network Organization during Cell Spreading.Cell reports · 2019Article
- Nanobody-CD16 Catch Bond Reveals NK Cell Mechanosensitivity.Biophysical journal · 2019Article
- Biphasic mechanosensitivity of T cell receptor-mediated spreading of lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- NK cells specifically TCR-dressed to kill cancer cells.EBioMedicine · 2019Article
- Simultaneous defeat of MCF7 and MDA-MB-231 resistances by a hypericin PDT-tamoxifen hybrid therapy.NPJ breast cancer · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Spreading of T cells on antigen presenting cells is a crucial initial step in immune response. Spreading occurs through rapid morphological changes concomitant with the reorganization of surface receptors and of the cytoskeleton. Ligand mobility and frictional coupling of receptors to the cytoskeleton were separately recognized as important factors but a systematic study to explore their biophysical role in spreading was hitherto missing. To explore the impact of ligand mobility, we prepared chemically identical substrates on which molecules of anti-CD3 (capable of binding and activating the T cell receptor complex), were either immobilized or able to diffuse. We quantified the T cell spreading area and cell edge dynamics using quantitative reflection interference contrast microscopy, and imaged the actin distribution. On mobile ligands, as compared to fixed ligands, the cells spread much less, the actin is centrally, rather than peripherally distributed and the edge dynamics is largely altered. Blocking myosin-II or adding molecules of ICAM1 on the substrate largely abrogates these differences. We explain these observations by building a model based on the balance of forces between activation-dependent actin polymerization and actomyosin-generated tension on one hand, and on the frictional coupling of the ligand-receptor complexes with the actin cytoskeleton, the membrane and the substrate, on the other hand. Introducing the measured edge velocities in the model, we estimate the coefficient of frictional coupling between T Cell receptors or LFA-1 and the actin cytoskeleton. Our results provide for the first time, to our knowledge, a quantitative framework bridging T cell-specific biology with concepts developed for integrin-based mechanisms of spreading.
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Registered trials
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