ArticleBiophysical journal2014
Role of suspended fiber structural stiffness and curvature on single-cell migration, nucleus shape, and focal-adhesion-cluster length.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Characterization of Collagen Fiber Organization in Breast Cancer via Model-Free Multiscale pSHG Image Analysis.Annals of biomedical engineering · 2026Article
- Profiling extracellular matrix-driven heterogeneity of single cell migration and morphology.Scientific reports · 2026Article
- The Interplay Between Stiffness and Hyperglycemia on Diabetic Foot Ulcer Wound Closure.Cellular and molecular bioengineering · 2026Article
- Quantifying cell traction forces at the single-fiber scale in 3D: An approach based on deformable photopolymerized fiber arrays.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Near-field electrospun 3D anisotropic fiber-hydrogel scaffold integrated with photothermal effect for skin wound healing.Bioactive materials · 2025Article
- Reinforcement of Fibrillar Collagen Hydrogels with Bioorthogonal Covalent Crosslinks.Biomacromolecules · 2025Article
- Dendritic cell force-migration coupling on aligned fiber networks.Biophysical journal · 2024Article
- Experimental and theoretical model for the origin of coiling of cellular protrusions around fibers.Nature communications · 2023Article
- What are the key mechanical mechanisms governing integrin-mediated cell migration in three-dimensional fiber networks?Biomechanics and modeling in mechanobiology · 2023Article
- Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.Biointerphases · 2023Article
- Actin Filaments Couple the Protrusive Tips to the Nucleus through the I-BAR Domain Protein IRSp53 during the Migration of Cells on 1D Fibers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Computational Analysis and Optimization of Geometric Parameters for Fibrous Scaffold Design.ACS omega · 2022Article
- Hydrogels for Salivary Gland Tissue Engineering.Gels (Basel, Switzerland) · 2022Review
- Sculpting Rupture-Free Nuclear Shapes in Fibrous Environments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Quantitative biophysical metrics for rapid evaluation of ovarian cancer metastatic potential.Molecular biology of the cell · 2022Article
- Biobased Elastomer Nanofibers Guide Light-Controlled Human-iPSC-Derived Skeletal Myofibers.Advanced materials (Deerfield Beach, Fla.) · 2022Article
- Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity.Signal transduction and targeted therapy · 2021Review
- Article
- Cell Fragment Formation, Migration, and Force Exertion on Extracellular Mimicking Fiber Nanonets.Advanced biology · 2021Article
- Single Cell Forces after Electroporation.ACS nano · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been shown that cellular migration, persistence, and associated cytoskeletal arrangement are highly dependent on substrate stiffness (modulus: N/m(2) and independent of geometry), but little is known on how cells respond to subtle changes in local geometry and structural stiffness (N/m). Here, using fibers of varying diameter (400, 700, and 1200 nm) and length (1 and 2 mm) deposited over hollow substrates, we demonstrate that single mouse C2C12 cells attached to single suspended fibers form spindle morphologies that are sensitive to fiber mechanical properties. Over a wide range of increasing structural stiffness (2 to 100+ mN/m), cells exhibited decreases in migration speed and average nucleus shape index of ∼57% (from 58 to 25 μm/h) and ∼26% (from 0.78 to 0.58), respectively, whereas the average paxillin focal-adhesion-cluster (FAC, formed at poles) length increased by ∼38% (from 8 to 11 μm). Furthermore, the increase in structural stiffness directly correlates with cellular persistence, with 60% of cells moving in the direction of increasing structural stiffness. At similar average structural stiffness (25 ± 5 mN/m), cells put out longer FAC lengths on smaller diameters, suggesting a conservation of FAC area, and also exhibited higher nucleus shape index and migration speeds on larger-diameter fibers. Interestingly, cells were observed to deform fibers locally or globally through forces applied through the FAC sites and cells undergoing mitosis were found to be attached to the FAC sites by single filamentous tethers. These varied reactions have implications in developmental and disease biology models as they describe a strong dependence of cellular behavior on the cell's immediate mechanistic environment arising from alignment and geometry of fibers.
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