ArticleJournal of nanobiotechnology2014
Toxicity of silver nanoparticle in rat ear and BALB/c 3T3 cell line.
Article in Journal of nanobiotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Therapeutic and Formulation Innovations in the Management of Canine Otitis Externa.Pharmaceutics · 2025Review
- Precision medicine: a new era for inner ear diseases.Frontiers in pharmacology · 2024Review
- Toxicological Aspects, Safety Assessment, and Green Toxicology of Silver Nanoparticles (AgNPs)-Critical Review: State of the Art.International journal of molecular sciences · 2023Review
- Biological Synthesis of Low Cytotoxicity Silver Nanoparticles (AgNPs) by the FungusJournal of fungi (Basel, Switzerland) · 2022Article
- NonToxic Silver/Poly-1-Vinyl-1,2,4-Triazole Nanocomposite Materials with Antibacterial Activity.Nanomaterials (Basel, Switzerland) · 2020Article
- High antiviral activity of mercaptoethane sulfonate functionalized Te/BSA nanostars against arterivirus and coronavirus.RSC advances · 2020Article
- Health Concerns of Various Nanoparticles: A Review of Their in Vitro and in Vivo Toxicity.Nanomaterials (Basel, Switzerland) · 2018Review
- Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After Intratympanic Administration.Nanoscale research letters · 2017Article
- Nanomedicine for Inner Ear Diseases: A Review of RecentBioMed research international · 2017Review
- Inner ear barriers to nanomedicine-augmented drug delivery and imaging.Journal of otology · 2016Review
- Involvement of Ubiquitin-Editing Protein A20 in Modulating Inflammation in Rat Cochlea Associated with Silver Nanoparticle-Induced CD68 Upregulation and TLR4 Activation.Nanoscale research letters · 2016Article
- Multilaboratory evaluation of 15 bioassays for (eco)toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID.Nanotoxicology · 2016Article
- Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection.Materials (Basel, Switzerland) · 2016Review
- Micro CT visualization of silver nanoparticles in the middle and inner ear of rat and transportation pathway after transtympanic injection.Journal of nanobiotechnology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSilver nanoparticles (AgNPs) displayed strong activities in anti-bacterial, anti-viral, and anti-fungal studies and was reportedly efficient in treating otitis media .The potential impact of AgNPs on the inner ear was missing.
objectiveAttempted to evaluate the potential toxicity of AgNPs in the inner ear, middle ear, and external ear canal after transtympanic injection in rats.
resultsIn in vitro studies, the IC50 for AgNPs in neutral red uptake assay was lower than that in NAD(P)H-dependent cellular oxidoreductase enzyme assay (WST-1) and higher than that in total cellular ATP and nuclear membrane integrity (propidium iodide) assessments. In in vivo experiments, magnetic resonance imaging (MRI) showed that significant changes in the permeability of biological barriers occurred in the middle ear mucosa, the skin of the external ear canal, and the inner ear at 5 h post-transtympanic injection of AgNPs at concentrations ranging from 20 μg/ml to 4000 μg/ml. The alterations in permeability showed a dosage-response relationship, and were reversible. The auditory brainstem response showed that 4000 μg/ml AgNPs induced hearing loss with partial recovery at 7 d, whereas 20 μg/ml caused reversible hearing loss. The functional change in auditory system was in line with the histology results. In general, the BALB/c 3T3 cell line is more than 1000 times more sensitive than the in vivo studies. Impairment of the mitochondrial function was indicated to be the mechanism of toxicity of AgNPs.
conclusionThese results suggest that AgNPs caused significant, dose-dependent changes in the permeability of biological barriers in the middle ear mucosa, the skin of the external ear canal, and the inner ear. In general, the BALB/c 3T3 cell line is more than 1000 times more sensitive than the in vivo studies. The rat ear model might be expended to other engineered nanomaterials in nanotoxicology study.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.