ArticleFunctional & integrative genomics2015
Double-stranded RNA in the biological control of grain aphid (Sitobion avenae F.).
Article in Functional & integrative genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Host-Induced Gene Silencing ofCurrent issues in molecular biology · 2026Article
- Exogenous application of nanocarrier-mediated double-stranded RNA manipulates physiological traits and defence response against bacterial diseases.Molecular plant pathology · 2024Article
- Article
- RNA Interference for Improving Disease Resistance in Plants and Its Relevance in This Clustered Regularly Interspaced Short Palindromic Repeats-Dominated Era in Terms of dsRNA-Based Biopesticides.Frontiers in plant science · 2022Review
- Silencing an aphid-specific geneFrontiers in plant science · 2022Article
- Review
- An RNAi supplemented diet as a reverse genetics tool to control bluegreen aphid, a major pest of legumes.Scientific reports · 2020Article
- The L-DOPA/Dopamine Pathway Transgenerationally Regulates Cuticular Melanization in the Pea AphidFrontiers in cell and developmental biology · 2020Article
- Silencing an essential gene involved in infestation and digestion in grain aphid through plant-mediated RNA interference generates aphid-resistant wheat plants.Plant biotechnology journal · 2019Article
- Host-Induced Gene Silencing: A Powerful Strategy to Control Diseases of Wheat and Barley.International journal of molecular sciences · 2019Review
- Improved annotation ofPeerJ · 2019Article
- RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants.International journal of molecular sciences · 2016Article
- RNA Interference in Insect Vectors for Plant Viruses.Viruses · 2016Review
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Grain aphid (Sitobion avenae F.) is the most dominant and destructive pest of wheat, which causes significant yield loss of cereal plants each year by inflicting damage both through the direct effects of feeding and by vectoring debilitating plant viruses. In this study, we performed de novo transcriptome sequencing of grain aphid via Roche 454 GS-FLX pyrosequencing. A total of 1,106,696 reads were obtained and assembled into 32,277 unigenes, of which 25,389, 21,635, and 16,211 unigenes matched the Nt, Nr, and Swiss-Prot databases, respectively. Functional annotation of these unigenes revealed not only the presence of genes that encode the key components of RNAi machinery such as Dicer and Argonaute but also the genes encoding the TAR RNA binding protein (TRBP) and the SID-1 protein, which function in assisting the RNA-induced silencing complex (RISC) formation in microRNA (miRNA) pathway and mediating a systemic RNA interference (RNAi) effect though a cellular uptake mechanism. Furthermore, among a set of 66 unigenes selected for a double-stranded RNA (dsRNA) artificial diet assay, four novel effective RNAi targets, which led to high mortality of aphids due to the down-regulation of the expression of the respective target gene, were identified. Moreover, the expansion of systemic RNAi effect in grain aphid was observed by adding the fluorescently labeled dsRNA in an artificial diet assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.