ArticleMolecular immunology2015
Synergistic effect of pro-inflammatory TNFα and IL-17 in periostin mediated collagen deposition: potential role in liver fibrosis.
Article in Molecular immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 69 citations in OpenAlex.
- Impact of Gene Polymorphism rs2275913 and Serum IL-17A Levels on Liver Fibrosis Severity Across the Natural History of Chronic Hepatitis B in Indonesia.Diseases (Basel, Switzerland) · 2026Article
- Glucolipid Metabolic Disorders andInternational journal of endocrinology · 2026Review
- Periostin regulates the activity of keloid fibroblasts by activating the JAK/STAT signaling pathway.Heliyon · 2024Article
- Targeting Interleukin-17 as a Novel Treatment Option for Fibrotic Diseases.Journal of clinical medicine · 2023Review
- Liver Regeneration and Immunity: A Tale to Tell.International journal of molecular sciences · 2023Review
- Th17 cells in the liver: balancing autoimmunity and pathogen defense.Seminars in immunopathology · 2022Review
- Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.Journal of nanobiotechnology · 2022Article
- Periostin in Angiogenesis and Inflammation in CRC-A Preliminary Observational Study.Medicina (Kaunas, Lithuania) · 2022Observational
- The role of interleukin-17 in the pathogenesis of systemic sclerosis: Pro-fibrotic or anti-fibrotic?Journal of scleroderma and related disorders · 2021Review
- Cytokines, paraoxonase-1, periostin and non-invasive liver fibrosis scores in patients with non-alcoholic fatty liver disease and persistently elevated aminotransferases: A pilot study.Experimental and therapeutic medicine · 2021Article
- Serum periostin and TNF-α levels in patients with obstructive sleep apnea-hypopnea syndrome.Sleep & breathing = Schlaf & Atmung · 2021Article
- Association of Pericardiac Adipose Tissue With Coronary Artery Disease.Frontiers in endocrinology · 2021Article
- Periostin: An Emerging Molecule With a Potential Role in Spinal Degenerative Diseases.Frontiers in medicine · 2021Review
- Novel Immune Subsets and Related Cytokines: Emerging Players in the Progression of Liver Fibrosis.Frontiers in medicine · 2021Review
- Extracellular vesicles-derived miR-150-5p secreted by adipose-derived mesenchymal stem cells inhibits CXCL1 expression to attenuate hepatic fibrosis.Journal of cellular and molecular medicine · 2021Article
- Periostin Circulating Levels and Genetic Variants in Patients with Non-Alcoholic Fatty Liver Disease.Diagnostics (Basel, Switzerland) · 2020Article
- Loss of IL-27Rα Results in Enhanced Tubulointerstitial Fibrosis Associated with Elevated Th17 Responses.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment.Frontiers in immunology · 2020Review
- miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma.BioMed research international · 2020Article
- IL-17 and TNF-α co-operation contributes to the proinflammatory response of hepatic stellate cells.Clinical and experimental immunology · 2019Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThe pro-inflammatory cytokines, tumor necrosis factor (TNF)-α, and interleukin (IL)-17, have been implicated in the pathogenesis of liver fibrosis. In this study, we investigated the role of TNFα and IL-17 toward induction of profibrotic factor, periostin.
methodsHepG2 cells were cultured and treated with inflammatory cytokines, TNFα and IL-17. Computational promoter sequence analysis of the periostin promoter was performed to define the putative binding sites for transcription factors. Transcription factors were analyzed by Western blot and Chromatin Immunoprecipitation. Periostin and transcription factor expression analysis was performed by RT-PCR, Western blot, and fluorescence microscopy. Type I collagen expression from fibroblast cultures was analyzed by Western blot and Sircol soluble collagen assay.
resultsActivation of HepG2 Cells with TNFα and IL-17 enhanced the expression of periostin (3.5 and 4.4 fold, respectively p<0.05) compared to untreated cells. However, combined treatment with both TNFα and IL-17 at similar concentration demonstrated a 13.3 fold increase in periostin (p<0.01), thus suggesting a synergistic role of these cytokines. Periostin promoter analysis and specific siRNA knock-down revealed that TNFα induces periostin through cJun, while IL-17 induced periostin via STAT-3 signaling mechanisms. Treatment of the supernatant from the cytokine activated HepG2 cells on fibroblast cultures induced enhanced expression of type I collagen (>9.1 fold, p<0.01), indicative of a direct fibrogenic effect of TNFα and IL-17.
conclusionTNFα and IL-17 induced fibrogenesis through cJun and STAT-3 mediated expression of profibrotic biomarker, periostin. Therefore, periostin might serve as a novel biomarker in early diagnosis of liver fibrosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.