ArticleAnalytica chimica acta2015
Glycoprofiling as a novel tool in serological assays of systemic sclerosis: a comparative study with three bioanalytical methods.
Article in Analytica chimica acta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- A lectin-coupled porous silicon-based biosensor: label-free optical detection of bacteria in a real-time mode.Scientific reports · 2020Article
- Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins.Bioelectrochemistry (Amsterdam, Netherlands) · 2017Article
- Full-length antibodies versus single-chain antibody fragments for a selective impedimetric lectin-based glycoprofiling of prostate specific antigen.Electrochimica acta · 2017Article
- Nanomaterial-based biosensors for detection of prostate specific antigen.Mikrochimica acta · 2017Review
- Graphene oxide-based electrochemical label-free detection of glycoproteins down to aM level using a lectin biosensor.The Analyst · 2016Article
- Mixed Zwitterion-Based Self-Assembled Monolayer Interface for Impedimetric Glycomic Analyses of Human IgG Samples in an Array Format.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Glycan and lectin biosensors.Essays in biochemistry · 2016Review
- Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- An ultrasensitive impedimetric glycan biosensor with controlled glycan density for detection of lectins and influenza hemagglutinins.Chemical communications (Cambridge, England) · 2015Article
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
Abstract
Systemic sclerosis (SSc) is an autoimmune disease seriously affecting patient's quality of life. The heterogeneity of the disease also means that identification and subsequent validation of biomarkers of the disease is quite challenging. A fully validated single biomarker for diagnosis, prognosis, disease activity and assessment of response to therapy is not yet available. The main aim of this study was to apply an alternative assay protocol to the immunoassay-based analysis of this disease by employment of sialic acid recognizing lectin Sambucus nigra agglutinin (SNA) to glycoprofile serum samples. To our best knowledge this is the first study describing direct lectin-based glycoprofiling of serum SSc samples. Three different analytical methods for glycoprofiling of serum samples relying on application of lectins are compared here from a bioanalytical point of view including traditional ELISA-like lectin-based method (ELLA), novel fluorescent lectin microarrays and ultrasensitive impedimetric lectin biosensors. Results obtained by all three bioanalytical methods consistently showed differences in the level of sialic acid present on glycoproteins, when serum from healthy people was compared to the one from patients having SSc. Thus, analysis of sialic acid content in human serum could be of a diagnostic value for future detection of SSc, but further work is needed to enhance selectivity of assays for example by glycoprofiling of a fraction of human serum enriched in antibodies for individual diagnostics.
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