ArticleGenome research2015
Defining heterochromatin in C. elegans through genome-wide analysis of the heterochromatin protein 1 homolog HPL-2.
Article in Genome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Nuclear envelope-dependent heterochromatin positioning gates the timing of nucleolar assembly in the early embryo.bioRxiv : the preprint server for biology · 2026Article
- Proximity labeling at H3K9me3 reveals VRK-1 regulate global chromatin distribution inbioRxiv : the preprint server for biology · 2026Article
- HPL-2/HP1 and MET-2/SETDB1 bind distinct co-factors that promote heterochromatic foci, gene repression and organogenesis independently of H3K9 methylation.bioRxiv : the preprint server for biology · 2026Article
- The chromatin remodeler LET-418/Mi-2 regulates the intracellular pathogen response in the C. elegans intestine.BMC genomics · 2025Article
- Chromatin and epigenetics in aging biology.Genetics · 2025Review
- Pervasive Conservation of Intron Number and Other Genetic Elements Revealed by a Chromosome-level Genome Assembly of the Hyper-polymorphic Nematode Caenorhabditis brenneri.Genome biology and evolution · 2025Article
- Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos.The EMBO journal · 2024Article
- Pervasive conservation of intron number and other genetic elements revealed by a chromosome-level genomic assembly of the hyper-polymorphic nematodebioRxiv : the preprint server for biology · 2024Article
- Chromatin Organization duringDNA · 2024Article
- Article
- Systematic characterization of chromodomain proteins reveals an H3K9me1/2 reader regulating aging in C. elegans.Nature communications · 2023Article
- H3K9me1/2 methylation limits the lifespan ofeLife · 2022Article
- Establishment of H3K9-methylated heterochromatin and its functions in tissue differentiation and maintenance.Nature reviews. Molecular cell biology · 2022Review
- The zinc-finger transcription factor LSL-1 is a major regulator of the germline transcriptional program in Caenorhabditis elegans.Genetics · 2022Article
- The Cell Biology of Heterochromatin.Cells · 2022Review
- SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity.Nature structural & molecular biology · 2022Article
- Gene bookmarking by the heat shock transcription factor programs the insulin-like signaling pathway.Molecular cell · 2021Article
- H3K9me selectively blocks transcription factor activity and ensures differentiated tissue integrity.Nature cell biology · 2021Article
- Region-specific H3K9me3 gain in aged somatic tissues in Caenorhabditis elegans.PLoS genetics · 2021Article
- Small RNAs and chromatin in the multigenerational epigenetic landscape ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
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4 authors.
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Abstract
Formation of heterochromatin serves a critical role in organizing the genome and regulating gene expression. In most organisms, heterochromatin flanks centromeres and telomeres. To identify heterochromatic regions in the heavily studied model C. elegans, which possesses holocentric chromosomes with dispersed centromeres, we analyzed the genome-wide distribution of the heterochromatin protein 1 (HP1) ortholog HPL-2 and compared its distribution to other features commonly associated with heterochromatin. HPL-2 binding highly correlates with histone H3 mono- and dimethylated at lysine 9 (H3K9me1 and H3K9me2) and forms broad domains on autosomal arms. Although HPL-2, like other HP1 orthologs, binds H3K9me peptides in vitro, the distribution of HPL-2 in vivo appears relatively normal in mutant embryos that lack H3K9me, demonstrating that the chromosomal distribution of HPL-2 can be achieved in an H3K9me-independent manner. Consistent with HPL-2 serving roles independent of H3K9me, hpl-2 mutant worms display more severe defects than mutant worms lacking H3K9me. HPL-2 binding is enriched for repetitive sequences, and on chromosome arms is anticorrelated with centromeres. At the genic level, HPL-2 preferentially associates with well-expressed genes, and loss of HPL-2 results in up-regulation of some binding targets and down-regulation of others. Our work defines heterochromatin in an important model organism and uncovers both shared and distinctive properties of heterochromatin relative to other systems.
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