ArticleGenetics2015
Variation in crossover frequencies perturb crossover assurance without affecting meiotic chromosome segregation in Saccharomyces cerevisiae.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Multiple independent losses of crossover interference during yeast evolutionary history.PLoS genetics · 2024Article
- Modeling Recombination Rate as a Quantitative Trait Reveals New Insight into Selection in Humans.Genome biology and evolution · 2023Article
- ThePNAS nexus · 2023Article
- Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltii.PLoS genetics · 2023Article
- A widespread inversion polymorphism conserved among Saccharomyces species is caused by recurrent homogenization of a sporulation gene family.PLoS genetics · 2022Article
- Diffusion and distal linkages govern interchromosomal dynamics during meiotic prophase.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Loss of Heterozygosity and Base Mutation Rates Vary AmongG3 (Bethesda, Md.) · 2020Article
- Regulated Proteolysis of MutSγ Controls Meiotic Crossing Over.Molecular cell · 2020Article
- Crossover Interference: Shedding Light on the Evolution of Recombination.Annual review of genetics · 2019Review
- Spore-autonomous fluorescent protein expression identifies meiotic chromosome mis-segregation as the principal cause of hybrid sterility in yeast.PLoS biology · 2018Article
- Review
- Modeling the genetic relatedness of Plasmodium falciparum parasites following meiotic recombination and cotransmission.PLoS computational biology · 2018Article
- Low recombination rates in sexual species and sex-asex transitions.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Genome Dynamics of HybridG3 (Bethesda, Md.) · 2017Article
- mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide.PLoS genetics · 2017Article
- Modulating Crossover Frequency and Interference for Obligate Crossovers inG3 (Bethesda, Md.) · 2017Article
- Evolutionary mysteries in meiosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Extensive Recombination of a Yeast Diploid Hybrid through Meiotic Reversion.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
The segregation of homologous chromosomes during the Meiosis I division requires an obligate crossover per homolog pair (crossover assurance). In Saccharomyces cerevisiae and mammals, Msh4 and Msh5 proteins stabilize Holliday junctions and its progenitors to facilitate crossing over. S. cerevisiae msh4/5 hypomorphs that reduce crossover levels up to twofold at specific loci on chromosomes VII, VIII, and XV without affecting homolog segregation were identified recently. We use the msh4-R676W hypomorph to ask if the obligate crossover is insulated from variation in crossover frequencies, using a S. cerevisiae S288c/YJM789 hybrid to map recombination genome-wide. The msh4-R676W hypomorph made on average 64 crossovers per meiosis compared to 94 made in wild type and 49 in the msh4Δ mutant confirming the defect seen at individual loci on a genome-wide scale. Crossover reductions in msh4-R676W and msh4Δ were significant across chromosomes regardless of size, unlike previous observations made at specific loci. The msh4-R676W hypomorph showed reduced crossover interference. Although crossover reduction in msh4-R676W is modest, 42% of the four viable spore tetrads showed nonexchange chromosomes. These results, along with modeling of crossover distribution, suggest the significant reduction in crossovers across chromosomes and the loss of interference compromises the obligate crossover in the msh4 hypomorph. The high spore viability of the msh4 hypomorph is maintained by efficient segregation of the natural nonexchange chromosomes. Our results suggest that variation in crossover frequencies can compromise the obligate crossover and also support a mechanistic role for interference in obligate crossover formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.