Evidence map›Paper›PMID 25466886›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015

Inhibition of cholinergic pathways in Drosophila melanogaster by α-conotoxins.

Mari D Heghinian, Monica Mejia, David J Adams, Tanja A Godenschwege, Frank Marí

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Mari D Heghinian*Department of Chemistry and Biochemistry and Department of Biological Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and Health Innovations Research Institute, RMIT University, Melbourne, Victoria, Australia.
Monica Mejia*Department of Chemistry and Biochemistry and Department of Biological Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and Health Innovations Research Institute, RMIT University, Melbourne, Victoria, Australia.
David J Adams*Department of Chemistry and Biochemistry and Department of Biological Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and Health Innovations Research Institute, RMIT University, Melbourne, Victoria, Australia.
Tanja A Godenschwege*Department of Chemistry and Biochemistry and Department of Biological Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and Health Innovations Research Institute, RMIT University, Melbourne, Victoria, Australia.
Frank Marí*Department of Chemistry and Biochemistry and Department of Biological Sciences, Florida Atlantic University, Boca Raton, Florida, USA; and Health Innovations Research Institute, RMIT University, Melbourne, Victoria, Australia mari@fau.edu.
Florida Atlantic University · USRMIT University · AU

Funding

Efficacious Screening of Peptidic Natural Products Using DrosophilaR21NS066371 · NINDS · FLORIDA ATLANTIC UNIVERSITY · PI MARI, FRANK · 2009 to 2010
$392k
NINDS NIH HHS R21NS06637NINDS NIH HHS R21 NS066371
6 · The paper itself

Abstract

Nicotinic acetylcholine receptors (nAChRs) play a pivotal role in synaptic transmission of neuronal signaling pathways and are fundamentally involved in neuronal disorders, including Alzheimer's disease, Parkinson's disease, and schizophrenia. In vertebrates, cholinergic pathways can be selectively inhibited by α-conotoxins; we show that in the model organism Drosophila, the cholinergic component of the giant fiber system is inhibited by α-conotoxins MII, AuIB, BuIA, EI, PeIA, and ImI. The injection of 45 pmol/fly of each toxin dramatically decreases the response of the giant fiber to dorsal longitudinal muscle (GF-DLM) connection to 20 ± 13.9% for MII; 26 ± 13.7% for AuIB, 12 ± 9.9% for BuIA, 30 ± 11.3% for EI, 1 ± 1% for PeIA, and 34 ± 15.4% for ImI. Through bioassay-guided fractionation of the venom of Conus brunneus, we found BruIB, an α-conotoxin that inhibits Drosophila nicotinic receptors but not its vertebrate counterparts. GF-DLM responses decreased to 43.7 ± 8.02% on injection of 45 pmol/fly of BruIB. We manipulated the Dα7 nAChR to mimic the selectivity of its vertebrate counterpart by placing structurally guided point mutations in the conotoxin-binding site. This manipulation rendered vertebrate-like behavior in the Drosophila system, enhancing the suitability of Drosophila as an in vivo tool to carry out studies related to human neuronal diseases. .

Indexed as

Acetylcholinealpha7 Nicotinic Acetylcholine ReceptorAmino Acid SequenceAnimalsAnimals, Genetically ModifiedBinding SitesCholinergic AgentsConotoxinsConus SnailDrosophila melanogasterFemaleGiant CellsHumansMaleModels, MolecularMolecular Sequence DataAcetylcholinealpha7 Nicotinic Acetylcholine Receptoralpha-conotoxin MIICholinergic AgentsConotoxinsNicotinic AntagonistsPeptide FragmentsConus brunneusgiant fiber systemnicotinic acetylcholine receptorssynaptic transmission

Identifiers

PMID25466886
PMCPMC4422358
OpenAlexW2009244491

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.