ArticleJournal of orthopaedic surgery and research2014
Fabrication and properties of acellular porcine anulus fibrosus for tissue engineering in spine surgery.
Article in Journal of orthopaedic surgery and research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Supercritical carbon dioxide decellularization of porcine annulus fibrosus for regenerative medicine.Journal of orthopaedic surgery and research · 2026Article
- Textile Design of an Intervertebral Disc Replacement Device from Silk Yarn.Biomimetics (Basel, Switzerland) · 2023Article
- Decellularized matrix for repairing intervertebral disc degeneration: Fabrication methods, applications and animal models.Materials today. Bio · 2023Review
- Putting the Pieces in Place: Mobilizing Cellular Players to Improve Annulus Fibrosus Repair.Tissue engineering. Part B, Reviews · 2021Review
- Decellularized Intervertebral Discs: A Potential Replacement for Degenerate Human Discs.Tissue engineering. Part C, Methods · 2020Article
- Optimized decellularization protocol including α-Gal epitope reduction for fabrication of an acellular porcine annulus fibrosus scaffold.Cell and tissue banking · 2017Article
- The Challenge in Using Mesenchymal Stromal Cells for Recellularization of Decellularized Cartilage.Stem cell reviews and reports · 2017Review
- Efficient decellularization for tissue engineering of the tendon-bone interface with preservation of biomechanics.PloS one · 2017Article
- Construction Strategy and Progress of Whole Intervertebral Disc Tissue Engineering.Orthopaedic surgery · 2016Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOver the last few years, new treatments for a damaged intervertebral disc (IVD) have included strategies to repair, replace, or regenerate the degenerative disc. However, these techniques are likely to have limited success, due to insufficiently effective means to address the damaged anulus fibrosus (AF). Here, we try to develop a bioprocess method for decellularization of the xenogeneic AF tissue, with a view to developing a scaffold as a potential candidate for clinical application in spinal surgery.
methodsPorcine AFs were decellularized using freeze-thaw cycles, followed by various combined treatments with 0.1% sodium dodecyl sulfate (SDS) and nucleases.
resultsHematoxylin and eosin (H & E) staining showed that decellularization was achieved through the decellularization protocols. Biochemical analyses revealed 86% reduction in DNA, but only 15.9% reduction in glycosaminoglycan (GAG) content, with no significant difference in the hydroxyproline content. There was no appreciable cytotoxicity of the acellular AF. Biomechanical testing of the acellular AF found no significant decline in stiffness or Young's modulus.
conclusionsPorcine AF tissues were effectively decellularized with the preservation of biologic composition and mechanical properties. These results demonstrate that acellular AF scaffolds would be a potential candidate for clinical application in spinal surgery.
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