ArticleScientific reports2014
AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discovery and genetic mapping.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
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- Recent Advances in Studies of Genomic DNA Methylation and Its Involvement in Regulating Drought Stress Response in Crops.Plants (Basel, Switzerland) · 2024Review
- The autotetraploid potato genome provides insights into highly heterozygous species.Plant biotechnology journal · 2022Article
- Hyper-seq: A novel, effective, and flexible marker-assisted selection and genotyping approach.Innovation (Cambridge (Mass.)) · 2022Article
- Genotyping by sequencing-based linkage map construction and identification of quantitative trait loci for yield-related traits and oil content in Jatropha (Jatropha curcas L.).Molecular biology reports · 2022Article
- Feature Compression Applications of Genetic Algorithm.Frontiers in genetics · 2022Article
- Genome-wide association analysis identified molecular markers associated with important tea flavor-related metabolites.Horticulture research · 2021Article
- Genome-Wide Association Mapping of Late Blight Tolerance Trait in Potato (Frontiers in genetics · 2021Article
- Cassava breeding and agronomy in Asia: 50 years of history and future directions.Breeding science · 2020Article
- Technological Innovations for Improving Cassava Production in Sub-Saharan Africa.Frontiers in genetics · 2020Review
- Article
- Construction of a high-density linkage map and QTL mapping for important agronomic traits in Stylosanthes guianensis (Aubl.) Sw.Scientific reports · 2019Article
- Transcription FactorFrontiers in plant science · 2019Article
- msgbsR: An R package for analysing methylation-sensitive restriction enzyme sequencing data.Scientific reports · 2018Article
- Genome-Wide Association Studies of 11 Agronomic Traits in Cassava (Frontiers in plant science · 2018Article
- Construction of an ultrahigh-density genetic linkage map forBiotechnology for biofuels · 2018Article
- Epigenetic map and genetic map basis of complex traits in cassava population.Scientific reports · 2017Article
- A High-Density SNP Genetic Linkage Map and QTL Analysis of Growth-Related Traits in a Hybrid Family of Oysters (Crassostrea gigas × Crassostrea angulata) Using Genotyping-by-Sequencing.G3 (Bethesda, Md.) · 2016Article
- Meristem micropropagation of cassava (Manihot esculenta) evokes genome-wide changes in DNA methylation.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaII) and a next-generation sequencing platform. Two sets of 85 adapter pairs were developed to concurrently identify SNPs, indels and methylation sites for 85 lines of cassava population in this study. In addition to SNPs and indels, the simplicity of the AFSM protocol makes it particularly suitable for high-throughput full methylation and hemi-methylation analyses. To further demonstrate the ease of this approach, a cassava genetic linkage map was constructed. This approach should be widely applicable for genetic mapping in a variety of organisms and will improve the application of crop genomics in assisted breeding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.