ArticleReproductive biology and endocrinology : RB&E2014
Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial.
Article in Reproductive biology and endocrinology : RB&E, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Novel Approach of Semen Preparation to Improve ICSI Outcome.
Evaluation Study Between Density Gradient and Swimming Down for Semen Processing
Who cites it
36 citing papers in PubMed, 89 citations in OpenAlex.
- A fast and safe technique for sperm preparation in ICSI treatments within a randomized controlled trial (RCT).Reproductive biology and endocrinology : RB&E · 2020Trial
- Microfluidic Intracytoplasmic Sperm Injection (MICSI): A Novel Platform for Sperm Isolation, Selection, and Injection Into Oocytes.Andrology · 2026Article
- Expression of miR-339-3p and OPRM1 in relation to sperm function in male infertility.Andrology · 2026Article
- Sperm DNA fragmentation and its influence on mammalian reproduction.Nature reviews. Urology · 2026Review
- Sequencing Matters: Comparative Effectiveness of Density-Gradient Centrifugation and MACS for Enhancing Sperm Quality and DNA Integrity.Journal of clinical medicine · 2026Article
- Functional heterogeneity of sperm binding to the ZP2 N terminus across normospermic and infertile phenotypes.Journal of translational medicine · 2026Article
- Bicarbonate buffer enhances functional sperm selection compared to Zwitterionic buffers in sperm preparation.Scientific reports · 2026Article
- Exploring the impact of the common (4977 bp) and ATPase mitochondrial DNA deletion on the copy number and telomere length in a sample of infertile Iraqi men.Molecular biology reports · 2026Article
- In vitro models of the male reproductive system: applications for developmental and reproductive toxicology.Toxicological sciences : an official journal of the Society of Toxicology · 2025Review
- Sperm preparation techniques affect biological parameters, fertility indices, and DNA fragmentation in patients with oligo-astheno-teratozoospermia.Reproduction & fertility · 2025Article
- Functional parameters of spermatozoa obtained by a new selection device.FEBS open bio · 2025Article
- Characterizing Extracellular Vesicles from Biological Fluids.Journal of visualized experiments : JoVE · 2025Article
- Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges.Animals : an open access journal from MDPI · 2025Review
- Comparative Efficacy of Swim-Up, Density-Gradient Centrifugation, and Microfluidic Sorting in Sperm Preparation, and the Impact on Motility, Morphology, and DNA Integrity.International journal of general medicine · 2025Article
- Enhanced sperm isolation via bulk acoustic waves for high-throughput motility screening.Scientific reports · 2024Article
- Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes.Animals : an open access journal from MDPI · 2024Article
- Evaluation of microRNA expression profiles in human sperm frozen using permeable cryoprotectant-free droplet vitrification and conventional methods.Asian journal of andrology · 2024Article
- Sperm DNA Fragmentation after Cryopreservation and Sperm Selection Has No Implications for Clinical Pregnancies and Live Births after Intrauterine Insemination with Donor Sperm.Journal of personalized medicine · 2023Article
- Enhancing the Accuracy of Measuring DEP Force Applied on Cells by Considering the Friction Effect.Biosensors · 2023Article
- Comparative sperm recovery rate after density gradient centrifugation with two media for in vitro fertilization.JBRA assisted reproduction · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDensity gradient is the preferred technique for sperm processing for ART. However, no study has examined sperm quality using different processing media simultaneously and under identical conditions. Therefore, we evaluated semen quality following sperm preparation by three commonly used commercially available density gradient media in a well-designed controlled trial.
methodsWe obtained semen samples from 20 healthy volunteers. Percent motility, total motile sperm (TMS), % recovery and DNA damage were assessed before and after separation in three different sperm density gradient media-PureCeption, ISolate and SpermGrad-125.
resultsPercent motility was higher in the ISolate (81.4% ± 6.6%) and SpermGrad-125 samples (85.7% ± 8.0%) (P < 0.0001) than in the PureCeption samples (62.5% ± 13.2%) (P = 0.07). TMS was higher in the PureCeption(TM) and ISolate samples (14.2% ± 15.9% and 15.8% ± 18.2%) than in those prepared with SpermGrad-125 (10.6% ± 19.7%) (P < 0.0001). Percent recovery was significantly higher in the PureCeption(TM) and ISolate samples (45.3% and 48.9%) than in the SpermGrad-125(TM) samples (30.8%) (P < 0.01). DNA fragmentation was comparable across the three gradients (PureCeption = 8.8% ± 4.7%; ISolate = 7.2 ± 5.2% and SpermGrad-125 = 11.2% ± 7.4%).
conclusionsThree different density gradient processing media PureCeption, ISolate, and SpermGrad-125 were examined for their effects on sperm quality. Sperm processed by ISolate and Sperm Grad 125 had better motility and TMS after processing. The extent of DNA damage was comparable in all three gradients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.