Evidence map›Paper›PMID 25466231›Full record

ArticleJournal of chromatographic science2015

Analysis of Major Withanolides in Physalis longifolia Nutt. by HPLC-PDA.

Cong-Mei Cao, Kelly Kindscher, Robert J Gallagher, Huaping Zhang, Barbara N Timmermann

Abstract readValidation Study
In one paragraph

Article in Journal of chromatographic science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Cong-Mei CaoDepartment of Medicinal Chemistry, University of Kansas, Lawrence, KS, USA.
Kelly KindscherKansas Biological Survey, University of Kansas, Lawrence, KS, USA.
Robert J GallagherDepartment of Medicinal Chemistry, University of Kansas, Lawrence, KS, USA.
Huaping ZhangDepartment of Medicinal Chemistry, University of Kansas, Lawrence, KS, USA.
Barbara N TimmermannDepartment of Medicinal Chemistry, University of Kansas, Lawrence, KS, USA btimmer@ku.edu.

Funding

Avance II 500 High Performance Digital NMR Spectrometer SystemS10RR024664 · NCRR · UNIVERSITY OF KANSAS LAWRENCE · PI VANDERVELDE, DAVID G · 2007 to 2007
$468k
400 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETERS10RR014767 · NCRR · UNIVERSITY OF KANSAS LAWRENCE · PI VANDERVELDE, DAVID G · 2001 to 2001
$346k
NCRR NIH HHS S10 RR014767NCRR NIH HHS S10 RR024664NCRR NIH HHS S10RR024664
6 · The paper itself

Abstract

An analytical method based on high-performance liquid chromatography-photodiode array detection was developed for the simultaneous determination of three anti-proliferative withanolides [withalongolide A ( 1: ), withaferin A ( 2: ) and withalongolide B ( 3: )] present in the aboveground biomass of the long-leaf groundcherry, Physalis longifolia. This method was achieved by biomass extraction followed by chromatographic separation on C18 column eluted with a gradient acetonitrile-water mobile phase. Calibration curves produced satisfactory linear regression (r(2) > 0.9995) for each examined sample. The method was also validated for accuracy, precision and limits of detection and quantification. Such an approach is applicable for the rapid detection and quantitative assessment of withanolides in various P. longifolia accessions.

Indexed as

AcetonitrilesAntineoplastic Agents, PhytogenicCalibrationChromatography, High Pressure LiquidLimit of DetectionPhysalisReproducibility of ResultsWithanolidesacetonitrileAcetonitrilesAntineoplastic Agents, Phytogenicwithaferin AWithanolides

Identifiers

PMID25466231
PMCPMC4592342

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.