ArticleThe Plant cell2014
C2-domain abscisic acid-related proteins mediate the interaction of PYR/PYL/RCAR abscisic acid receptors with the plasma membrane and regulate abscisic acid sensitivity in Arabidopsis.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 58 papers, 1 of them a synthesis that pooled it.
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58 citing papers in PubMed, 1 synthesis or guideline pooled it, 149 citations in OpenAlex.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
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- Integrated genomic and transcriptomic analysis of maize SRC2-like genes highlights divergent roles in abiotic stress responses.Frontiers in plant science · 2026Article
- Identification of regulatory genes involved in anthocyanin accumulation in radish leaves using bulk segregant analysis.Scientific reports · 2025Article
- An intracellular CPK-ECA1 phosphoregulatory circuit couples calcium signatures to ABA homeostasis for plant osmosensivity.Science advances · 2025Article
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- Identification of peptidome-based biomarkers of cassava mosaic disease resistance in different cassava varieties.Scientific reports · 2025Article
- ABA-activated low-nanomolar CaNature plants · 2025Article
- The role of gibberellin synthase gene VvGA2ox7 acts as a positive regulator to salt stress in Arabidopsis thaliana.BMC plant biology · 2024Article
- Genome-Wide Transcriptional Response of Avocado toPlants (Basel, Switzerland) · 2024Article
- Functional Identification of MhPYL4 Involved in Iron-Deficiency Stress in Malus Halliana Koehne.Plants (Basel, Switzerland) · 2024Article
- Computational Reconstruction of the Transcription Factor Regulatory Network Induced by Auxin inPlants (Basel, Switzerland) · 2024Article
- Signatures of Adaptation and Purifying Selection in Highland Populations of Dasiphora fruticosa.Molecular biology and evolution · 2024Article
- PbHsfC1a-coordinates ABA biosynthesis and HPlant biotechnology journal · 2024Article
- Dual transcriptomic analysis reveals early inducedFrontiers in plant science · 2024Article
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- Aspartyl tRNA-synthetase (AspRS) gene family enhances drought tolerance in poplar through BABA-PtrIBIs-PtrVOZ signaling module.BMC genomics · 2023Article
- Genome-Wide Analysis of the FBA Subfamily of the Poplar F-Box Gene Family and Its Role under Drought Stress.International journal of molecular sciences · 2023Article
- The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis.Frontiers in plant science · 2023Article
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Authors and funding
13 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane-delimited abscisic acid (ABA) signal transduction plays a critical role in early ABA signaling, but the molecular mechanisms linking core signaling components to the plasma membrane are unclear. We show that transient calcium-dependent interactions of PYR/PYL ABA receptors with membranes are mediated through a 10-member family of C2-domain ABA-related (CAR) proteins in Arabidopsis thaliana. Specifically, we found that PYL4 interacted in an ABA-independent manner with CAR1 in both the plasma membrane and nucleus of plant cells. CAR1 belongs to a plant-specific gene family encoding CAR1 to CAR10 proteins, and bimolecular fluorescence complementation and coimmunoprecipitation assays showed that PYL4-CAR1 as well as other PYR/PYL-CAR pairs interacted in plant cells. The crystal structure of CAR4 was solved, which revealed that, in addition to a classical calcium-dependent lipid binding C2 domain, a specific CAR signature is likely responsible for the interaction with PYR/PYL receptors and their recruitment to phospholipid vesicles. This interaction is relevant for PYR/PYL function and ABA signaling, since different car triple mutants affected in CAR1, CAR4, CAR5, and CAR9 genes showed reduced sensitivity to ABA in seedling establishment and root growth assays. In summary, we identified PYR/PYL-interacting partners that mediate a transient Ca(2+)-dependent interaction with phospholipid vesicles, which affects PYR/PYL subcellular localization and positively regulates ABA signaling.
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