Evidence map›Paper›PMID 25465114›Full record

ArticleCell stem cell2015

GPI-80 defines self-renewal ability in hematopoietic stem cells during human development.

Sacha Leandra Prashad, Vincenzo Calvanese, Catherine Yao Yao, Joshua Kaiser, Yanling Wang, Rajkumar Sasidharan, Gay Crooks, Mattias Magnusson, Hanna Katri Annikki Mikkola

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stem cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 74 citations in OpenAlex.

  1. Cannabidiol and ΔToxicology · 2026
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  9. The emergence of the stem cell niche.Trends in cell biology · 2023
    Review
  10. Hematopoietic Stem Cell Development in Mammalian Embryos.Advances in experimental medicine and biology · 2023
    Review
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  12. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Sacha Leandra PrashadDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Vincenzo CalvaneseDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Catherine Yao YaoDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Joshua KaiserDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Yanling WangDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Rajkumar SasidharanDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Gay CrooksDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Mattias MagnussonDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Hanna Katri Annikki MikkolaDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: hmikkola@mcdb.ucla.edu.
Broad Center · USUniversity of California, Los Angeles · US

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Transduction of Human Stem and Progenitor CellsP01HL073104 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KOHN, DONALD B · 2003 to 2013
$18.3M
Defining the Self-Renewal Program in Human Hematopoietic Stem CellsR01DK100959 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MIKKOLA, HANNA KATRI ANNIKKI · 2014 to 2022
$2.9M
PDGF-B signaling in fetal hematopoietic stem cell microenvironmentsR01HL097766 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MIKKOLA, HANNA KATRI ANNIKKI · 2009 to 2012
$1.5M
Defining the Self-Renewal Program in Human Hematopoietic Stem CellsR56DK100959 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MIKKOLA, HANNA KATRI ANNIKKI · 2017 to 2017
$226k
Howard Hughes Medical InstituteNCI NIH HHS P30 CA016042NHLBI NIH HHS HL097766NHLBI NIH HHS P01 HL073104NHLBI NIH HHS R01 HL097766NIDDK NIH HHS DK100959NIDDK NIH HHS R01 DK100959NIDDK NIH HHS R56 DK100959
6 · The paper itself

Abstract

Advances in pluripotent stem cell and reprogramming technologies have given us the hope of generating hematopoietic stem cells (HSCs) in culture. To succeed, greater understanding of the self-renewing HSC during human development is required. We discovered that the glycophosphatidylinositol-anchored surface protein GPI-80 defines a subpopulation of human fetal liver hematopoietic stem/progenitor cells (HSPCs) with self-renewal ability. CD34(+)CD38(lo/-)CD90(+)GPI-80(+) HSPCs were the sole population that maintained proliferative potential and an undifferentiated state in stroma coculture and engrafted in immunodeficient mice. GPI-80 expression also enabled tracking of HSPCs once they emerged from endothelium and migrated between human fetal hematopoietic niches. GPI-80 colocalized on the surface of HSPCs with Integrin alpha-M (ITGAM), which in leukocytes cooperates with GPI-80 to support migration. Knockdown of GPI-80 or ITGAM was sufficient to compromise HSPC expansion in culture and engraftment in vivo. These findings indicate that human fetal HSCs employ mechanisms used in leukocyte adhesion and migration to mediate HSC self-renewal.

Indexed as

Embryonic DevelopmentAmidohydrolasesCD11b AntigenCell Adhesion MoleculesCell DifferentiationCell LineageCell ProliferationFetusFlow CytometryGPI-Linked ProteinsHematopoietic Stem CellsHematopoietic Stem Cell TransplantationHumansImmunophenotypingLiverAmidohydrolasesCD11b AntigenCell Adhesion MoleculesGPI-Linked ProteinsITGAM protein, humanVNN2 protein, human

Identifiers

PMID25465114
PMCPMC4520393
OpenAlexW2006816191

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.