ArticleGenome biology2014
Tomato nuclear proteome reveals the involvement of specific E2 ubiquitin-conjugating enzymes in fruit ripening.
Article in Genome biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 115 citations in OpenAlex.
- Characterization and expression analysis of UBC gene family provide insights into the potential roles in pigment biosynthesis in pepper fruit.BMC genomics · 2026Article
- Unravelling the genetics of heat-responsive fruit traits in pepper [Capsicum annuum L.] via GWAS under subtropical climates.BMC plant biology · 2026Article
- Molecular mechanisms of ABA-mediated cold stress response in Dendrobium officinale.BMC plant biology · 2026Article
- Protein post-translational modifications: key switches coordinating fruit ripening regulatory networks.Horticulture research · 2026Article
- SlAREB1 regulates ethylene biosynthesis and mediates the effect of abscisic acid on postharvest ripening of tomato fruit.Frontiers in plant science · 2026Article
- SC35-mediated bZIP49 splicing regulates K⁺ channel AKT1 for salt stress adaptation in poplar.Nature communications · 2025Article
- The MADS-Box Transcription Factor CaRIN Positively Regulates Chlorophyll Degradation During Pepper (Plants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- The transcription factor PbbHLH164 is destabilized by PbRAD23C/D.1 and mediates ethylene biosynthesis during pear fruit ripening.Journal of advanced research · 2024Article
- A NAC triad modulates plant immunity by negatively regulating N-hydroxy pipecolic acid biosynthesis.Nature communications · 2024Article
- Genome‑wide identification and expression analysis of the UBC gene family in wheat (Triticum aestivum L.).BMC plant biology · 2024Article
- Article
- MaNAC029 modulates ethylene biosynthesis and fruit quality and undergoes MaXB3-mediated proteasomal degradation during banana ripening.Journal of advanced research · 2023Article
- Deciphering the regulatory network of the NAC transcription factor FvRIF, a key regulator of strawberry (Fragaria vesca) fruit ripening.The Plant cell · 2023Article
- Current insights into posttranscriptional regulation of fleshy fruit ripening.Plant physiology · 2023Review
- ChIP-Seq Analysis ofFoods (Basel, Switzerland) · 2023Article
- An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice.The Plant cell · 2023Article
- The Ubiquitin-26S Proteasome Pathway and Its Role in the Ripening of Fleshy Fruits.International journal of molecular sciences · 2023Review
- Genome-wide characterization of ubiquitin-conjugating enzyme gene family explores its genetic effects on the oil content and yield ofFrontiers in plant science · 2023Article
- Epigenetic regulation in tomato fruit ripening.Frontiers in plant science · 2023Review
3 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFruits are unique to flowering plants and play a central role in seed maturation and dispersal. Molecular dissection of fruit ripening has received considerable interest because of the biological and dietary significance of fruit. To better understand the regulatory mechanisms underlying fruit ripening, we report here the first comprehensive analysis of the nuclear proteome in tomato fruits.
resultsNuclear proteins were isolated from tomatoes in different stages of ripening, and subjected to iTRAQ (isobaric tags for relative and absolute quantification) analysis. We show that the proteins whose abundances change during ripening stages are involved in various cellular processes. We additionally evaluate changes in the nuclear proteome in the ripening-deficient mutant, ripening-inhibitor (rin), carrying a mutation in the transcription factor RIN. A set of proteins were identified and particular attention was paid to SlUBC32 and PSMD2, the components of ubiquitin-proteasome pathway. Through chromatin immunoprecipitation and gel mobility shift assays, we provide evidence that RIN directly binds to the promoters of SlUBC32 and PSMD2. Moreover, loss of RIN function affects protein ubiquitination in nuclei. SlUBC32 encodes an E2 ubiquitin-conjugating enzyme and a genome-wide survey of the E2 gene family in tomatoes identified five more E2s as direct targets of RIN. Virus-induced gene silencing assays show that two E2s are involved in the regulation of fruit ripening.
conclusionsOur results uncover a novel function of protein ubiquitination, identifying specific E2s as regulators of fruit ripening. These findings contribute to the unraveling of the gene regulatory networks that control fruit ripening.
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