Evidence map›Paper›PMID 25464144›Full record

ArticleMicron (Oxford, England : 1993)2015

A correlative optical microscopy and scanning electron microscopy approach to locating nanoparticles in brain tumors.

Paul J Kempen, Moritz F Kircher, Adam de la Zerda, Cristina L Zavaleta, Jesse V Jokerst, Ingo K Mellinghoff, Sanjiv S Gambhir, Robert Sinclair

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micron (Oxford, England : 1993), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
5.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Future Prospects for Clinical Applications of Nanocarbons Focusing on Carbon Nanotubes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Paul J KempenDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305-4034, USA. Electronic address: pkempen@stanford.edu.
Moritz F KircherMolecular Imaging Program at Stanford, Department of Radiology, Stanford University, Stanford, CA 94305-5427, USA; Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Department of Radiology, Weill Cornell Medical College, New York, NY 10021, USA; Brain Tumor Center, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Center for Molecular Imaging and Nanotechnology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Adam de la ZerdaMolecular Imaging Program at Stanford, Department of Radiology, Stanford University, Stanford, CA 94305-5427, USA; Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA; Department of Structural Biology, Stanford University, Stanford, CA 94305, USA.
Cristina L ZavaletaMolecular Imaging Program at Stanford, Department of Radiology, Stanford University, Stanford, CA 94305-5427, USA.
Jesse V JokerstMolecular Imaging Program at Stanford, Department of Radiology, Stanford University, Stanford, CA 94305-5427, USA.
Ingo K MellinghoffDepartment of Neurology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Human Oncology & Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Department of Pharmacology, Weill Cornell Medical College, New York, NY 10021, USA.
Sanjiv S GambhirDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305-4034, USA; Molecular Imaging Program at Stanford, Department of Radiology, Stanford University, Stanford, CA 94305-5427, USA; Department of Bio-engineering and Bio-X Program, Stanford University, Stanford, CA 94305-5427, USA.
Robert SinclairDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305-4034, USA.
Stanford University · USMemorial Sloan Kettering Cancer Center · USStanford Medicine · US

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Elisa de Stanchina · 1985 to 2026
$347.4M
Stanford Nanocharacterization LaboratoryU54CA119367 · NCI · STANFORD UNIVERSITY · PI GAMBHIR, SANJIV S · 2006 to 2010
$19.8M
Pilot ProjectsU54CA151459 · NCI · STANFORD UNIVERSITY · PI QUAKE, STEPHEN R · 2010 to 2014
$12.6M
Molecular Mechanisms of IDH1-mediated GliomagenesisR01NS080944 · NINDS · SLOAN-KETTERING INST CAN RESEARCH · PI MELLINGHOFF, INGO K · 2012 to 2016
$2.0M
MRI-Photoacoustic-Raman Nanostars for Pre- and Intraoperative Multimodal ImagingR01EB017748 · NIBIB · SLOAN-KETTERING INST CAN RESEARCH · PI KIRCHER, MORITZ FLORIAN · 2014 to 2017
$1.6M
Combined Pre & Intraoperative Brain Tumor Imaging Strategy using a Dual-ModalityK08CA163961 · NCI · SLOAN-KETTERING INST CAN RESEARCH · PI KIRCHER, MORITZ FLORIAN · 2012 to 2016
$845k
NCI NIH HHS K08 CA163961NCI NIH HHS P30 CA008748NCI NIH HHS U54 CA119367NCI NIH HHS U54 CA151459NCI NIH HHS U54CA151459NIBIB NIH HHS R01 EB017748NINDS NIH HHS R01 NS080944
6 · The paper itself

Abstract

The growing use of nanoparticles in biomedical applications, including cancer diagnosis and treatment, demands the capability to exactly locate them within complex biological systems. In this work a correlative optical and scanning electron microscopy technique was developed to locate and observe multi-modal gold core nanoparticle accumulation in brain tumor models. Entire brain sections from mice containing orthotopic brain tumors injected intravenously with nanoparticles were imaged using both optical microscopy to identify the brain tumor, and scanning electron microscopy to identify the individual nanoparticles. Gold-based nanoparticles were readily identified in the scanning electron microscope using backscattered electron imaging as bright spots against a darker background. This information was then correlated to determine the exact location of the nanoparticles within the brain tissue. The nanoparticles were located only in areas that contained tumor cells, and not in the surrounding healthy brain tissue. This correlative technique provides a powerful method to relate the macro- and micro-scale features visible in light microscopy with the nanoscale features resolvable in scanning electron microscopy.

Indexed as

NanoparticlesAnimalsBrain NeoplasmsDisease Models, AnimalMiceMicroscopyOptical ImagingCancer diagnosisCorrelative microscopyNanotechnologyOptical microscopyScanning electron microscopy

Identifiers

PMID25464144
PMCPMC4262686
OpenAlexW1977404636

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.