ArticleActa biomaterialia2015
Multilayer three-dimensional filter paper constructs for the culture and analysis of aortic valvular interstitial cells.
Article in Acta biomaterialia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 60 citations in OpenAlex.
- Investigation of oxidized low-density lipoprotein (oxLDL)-induced inflammation in atherosclerosis using a paper-based immune-vascular model.Analytical and bioanalytical chemistry · 2026Article
- Toward Origami-Inspired In Vitro Cardiac Tissue Models.ACS biomaterials science & engineering · 2025Article
- Optically Active, Paper-Based Scaffolds for 3D Cardiac Pacing.ACS applied materials & interfaces · 2024Article
- Models for calcific aortic valve disease in vivo and in vitro.Cell regeneration (London, England) · 2024Review
- Multiscale embedded printing of engineered human tissue and organ equivalents.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Recent Advances on Cell Culture Platforms for In Vitro Drug Screening and Cell Therapies: From Conventional to Microfluidic Strategies.Advanced healthcare materials · 2023Review
- A Hybrid Nanofiber/Paper Cell Culture Platform for Building a 3D Blood-brain Barrier Model.Small methods · 2021Article
- Unconventional Biomaterials for Cardiovascular Tissue Engineering.Current opinion in biomedical engineering · 2021Article
- Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform.Molecules (Basel, Switzerland) · 2020Article
- Biomedical Application of Functional Materials in Organ-on-a-Chip.Frontiers in bioengineering and biotechnology · 2020Review
- Tissue Papers: Leveraging Paper-Based Microfluidics for the Next Generation of 3D Tissue Models.Analytical chemistry · 2019Article
- Separation and Characterization of Prostate Cancer Cell Subtype according to Their Motility Using a Multi-Layer CiGiP Culture.Micromachines · 2018Article
- Valve Interstitial Cells: The Key to Understanding the Pathophysiology of Heart Valve Calcification.Journal of the American Heart Association · 2017Review
- Differential cell-matrix responses in hypoxia-stimulated aortic versus mitral valves.Journal of the Royal Society, Interface · 2016Article
- Cell culture platform fabrication methods and applications.Tzu chi medical journalReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Culturing aortic valvular interstitial cells in an environment that models the aortic valve is an essential step towards understanding the progression of calcific aortic valve disease. Here the adaption of a three-dimensional (3-D) stacked paper-based culture system is presented for analyzing valve cells in a thick collagen gel matrix. Filter paper layers, modeled after a 96-well plate design, were printed with a wax well-plate template and then seeded with valve cell and collagen mixtures that quickly gelled into 3-D cultures. Stacking these layers permitted extensive customization of culture thickness and cell density profiles to model the full thickness of native valve tissue. Aortic valvular interstitial cells seeded into the paper-based constructs consistently demonstrated high survival up to 14 days of culture with significant increases in cell number through the first 3 days of culture. After 4 days following seeding, valve cells in single layer cultures showed reduced smooth muscle α-actin expression with a stabilized cell density, suggesting a transition from an activated phenotype to a more quiescent state. Valve cells in multilayer cultures demonstrated the ability to migrate from layer to layer and had the highest smooth muscle α-actin expression in areas with predicted low oxygen tensions. These results establish the filter-paper-based method as a viable culture system for analyzing valve cells in an in vitro 3-D model of the aortic valve.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.