ArticleActa biomaterialia2015
Biphasic response of cell invasion to matrix stiffness in three-dimensional biopolymer networks.
Article in Acta biomaterialia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 136 citations in OpenAlex.
- Cell-Extracellular Matrix Feedback Results in Spontaneous Cell Polarization and Heterogeneous Remodeling in 3D Isotropic and Aligned Discrete-Fiber Models of Cell-Mediated Remodeling.Cellular and molecular bioengineering · 2026Article
- Effects of Intracellular Force Localization on Cancer Cell Invasion: Revealing Mechanical Trade-offs through Experimentally Validated Computational Models.ACS biomaterials science & engineering · 2026Article
- Extracellular matrix physical properties regulate cancer cell morphological transitions in 3D hydrogel microtissues.Acta biomaterialia · 2026Article
- Measurement of cellular traction forces during confined migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Extracellular matrix stiffness in endometrial cancer: driving progression and modulating treatment sensitivity via the ROCK1/YAP1 axis.Cell death & disease · 2025Article
- Matrix Stiffness-Mediated DNA Methylation in Endothelial Cells.Cellular and molecular bioengineering · 2025Article
- Modelling the Impact of HIF on Metabolism and the Extracellular Matrix: Consequences for Tumour Growth and Invasion.Bulletin of mathematical biology · 2025Article
- Insights into the mechanisms, regulation, and therapeutic implications of extracellular matrix stiffness in cancer.Bioengineering & translational medicine · 2025Review
- Solid stress compression enhances breast cancer cell migration through the upregulation of Interleukin-6.Frontiers in cell and developmental biology · 2025Article
- The Influence of Sulfation Degree of Glycosaminoglycan-Functionalized 3D Collagen I Networks on Cytokine Profiles of In Vitro Macrophage-Fibroblast Cocultures.Gels (Basel, Switzerland) · 2024Article
- Using Chemistry To Recreate the Complexity of the Extracellular Matrix: Guidelines for Supramolecular Hydrogel-Cell Interactions.Journal of the American Chemical Society · 2024Review
- Conductive Polyisocyanide Hydrogels Inhibit Fibrosis and Promote Myogenesis.ACS applied bio materials · 2024Article
- Natural Killer Cell Mechanosensing in Solid Tumors.Bioengineering (Basel, Switzerland) · 2024Review
- Optimal cell traction forces in a generalized motor-clutch model.Biophysical journal · 2023Article
- Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletion.BMC biology · 2023Article
- A Beginner's Guide to the Characterization of Hydrogel Microarchitecture for Cellular Applications.Gels (Basel, Switzerland) · 2022Review
- The Extracellular Matrix Stiffening: A Trigger of Prostate Cancer Progression and Castration Resistance?Cancers · 2022Review
- WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues.Developmental cell · 2022Article
- 3D modeling in cancer studies.Human cell · 2022Review
- Dissecting and Reconstructing Matrix in Malignant Mesothelioma Through Histocell-Histochemistry Gradients for Clinical Applications.Frontiers in medicine · 2022Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
When cells come in contact with an adhesive matrix, they begin to spread and migrate with a speed that depends on the stiffness of the extracellular matrix. On a flat surface, migration speed decreases with matrix stiffness mainly due to an increased stability of focal adhesions. In a three-dimensional (3-D) environment, cell migration is thought to be additionally impaired by the steric hindrance imposed by the surrounding matrix. For porous 3-D biopolymer networks such as collagen gels, however, the effect of matrix stiffness on cell migration is difficult to separate from effects of matrix pore size and adhesive ligand density, and is therefore unknown. Here we used glutaraldehyde as a crosslinker to increase the stiffness of self-assembled collagen biopolymer networks independently of collagen concentration or pore size. Breast carcinoma cells were seeded onto the surface of 3-D collagen gels, and the invasion depth was measured after 3 days of culture. Cell invasion in gels with pore sizes >5 μm increased with higher gel stiffness, whereas invasion in gels with smaller pores decreased with higher gel stiffness. These data show that 3-D cell invasion is enhanced by higher matrix stiffness, opposite to cell behavior in two dimensions, as long as the pore size does not fall below a critical value where it causes excessive steric hindrance. These findings may be important for optimizing the recellularization of soft tissue implants or for the design of 3-D invasion models in cancer research.
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