ArticlePloS one2014
Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Droplet based microfluidic and miniaturization at high-throughput level to screening heterologous secondary metabolites in microbes: A critical review.Biotechnology letters · 2025Review
- Isolation of xylanase-producing yeastFood science and biotechnology · 2025Article
- Sugar Beet Pulp as a Biorefinery Substrate for Designing Feed.Molecules (Basel, Switzerland) · 2023Article
- Review
- Yeast Protein as an Easily Accessible Food Source.Metabolites · 2022Review
- Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin.Applied microbiology and biotechnology · 2021Review
- Disruption of theFrontiers in microbiology · 2021Article
- Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme fromBiotechnology for biofuels · 2020Article
- Article
- Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica.Microbial cell factories · 2019Review
- Organic Wastes as Feedstocks for Non-Conventional Yeast-Based Bioprocesses.Microorganisms · 2019Review
- Enhanced protopanaxadiol production from xylose by engineered Yarrowia lipolytica.Microbial cell factories · 2019Article
- Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-AccumulatingFrontiers in microbiology · 2018Article
- Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi.Microbial cell factories · 2017Article
- Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica.Microbial cell factories · 2017Article
- Expressing accessory proteins in cellulolyticBiotechnology for biofuels · 2017Article
- Integrating Cellular and Bioprocess Engineering in the Non-Conventional YeastFrontiers in bioengineering and biotechnology · 2017Review
- Fatty alcohol production inBiotechnology for biofuels · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a Y. lipolytica strain able to degrade cellulose, in this study, we present successful expression of several xylanases in Y. lipolytica. The XynII and XlnD expressing Yarrowia strains exhibited an ability to grow on xylan mineral plates. This was shown by Congo Red staining of halo zones on xylan mineral plates. Enzymatic activity tests further demonstrated active expression of XynII and XlnD in Y. lipolytica. Furthermore, synergistic action in converting xylan to xylose was observed when XlnD acted in concert with XynII. The successful expression of these xylanases in Yarrowia further advances us toward our goal to develop a direct microbial conversion process using this organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.