ArticleRapid communications in mass spectrometry : RCM2015
Automated annotation and quantitation of glycans by liquid chromatography/electrospray ionization mass spectrometric analysis using the MultiGlycan-ESI computational tool.
Article in Rapid communications in mass spectrometry : RCM, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Qualitative Characterization of Neu5Gc-Containing N-Glycans in Commercial Cheddar Cheese Products Using Nano-UHPLC-ESI-MS/MS-A Pilot Study.International journal of molecular sciences · 2026Article
- Altered Cell SurfaceJournal of proteome research · 2025Article
- Glycomics in Human Diseases and Its Emerging Role in Biomarker Discovery.Biomedicines · 2025Review
- Recent advances in analytical methods and bioinformatic tools for quantitative glycomics.Analytical and bioanalytical chemistry · 2025Review
- Review
- GlycanGUI: Automated Glycan Annotation and Quantification Using Glucose Unit Index.Frontiers in chemistry · 2021Article
- gQuant, an Automated Tool for Quantitative Glycomic Data Analysis.Frontiers in chemistry · 2021Article
- 8-plex LC-MS/MS Analysis of PermethylatedAnalytical chemistry · 2019Article
- Structures and functions of invertebrate glycosylation.Open biology · 2019Review
- LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder.Electrophoresis · 2018Article
- Article
- Capillary electrophoresis-mass spectrometry for direct structural identification of serum N-glycans.Journal of chromatography. A · 2017Article
- Characterization of isomeric glycan structures by LC-MS/MS.Electrophoresis · 2017Review
- Glycomic and glycoproteomic analysis of glycoproteins-a tutorial.Analytical and bioanalytical chemistry · 2017Article
- Analysis of Permethylated Glycan by Liquid Chromatography (LC) and Mass Spectrometry (MS).Methods in molecular biology (Clifton, N.J.) · 2017Article
- A Brief Review of Bioinformatics Tools for Glycosylation Analysis by Mass Spectrometry.Mass spectrometry (Tokyo, Japan) · 2017Article
- Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach.Methods in enzymology · 2017Article
- Reliable LC-MS quantitative glycomics using iGlycoMab stable isotope labeled glycans as internal standards.Electrophoresis · 2016Article
- Deciphering glycomics and neuroproteomic alterations in experimental traumatic brain injury: Comparative analysis of aspirin and clopidogrel treatment.Electrophoresis · 2016Article
- LC-MS/MS analysis of permethylated free oligosaccharides and N-glycans derived from human, bovine, and goat milk samples.Electrophoresis · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
rationaleLiquid chromatography/mass spectrometry (LC/MS) is currently considered to be a conventional glycomics analysis strategy due to the high sensitivity and ability to handle complex biological samples. Interpretation of LC/MS data is a major bottleneck in high-throughput glycomics LC/MS-based analysis. The complexity of LC/MS data associated with biological samples prompts the needs to develop computational tools capable of facilitating automated data annotation and quantitation.
methodsAn LC/MS-based automated data annotation and quantitation software, MultiGlycan-ESI, was developed and utilized for glycan quantitation. Data generated by the software from LC/MS analysis of permethylated N-glycans derived from fetuin were initially validated by manual integration to assess the performance of the software. The performance of MultiGlycan-ESI was then assessed for the quantitation of permethylated fetuin N-glycans analyzed at different concentrations or spiked with permethylated N-glycans derived from human blood serum.
resultsThe relative abundance differences between data generated by the software and those generated by manual integration were less than 5%, indicating the reliability of MultiGlycan-ESI in quantitation of permethylated glycans analyzed by LC/MS. Automated quantitation resulted in a linear relationship for all six N-glycans derived from 50 ng to 400 ng fetuin with correlation coefficients (R(2) ) greater than 0.93. Spiking of permethylated fetuin N-glycans at different concentrations in permethylated N-glycan samples derived from a 0.02 μL of HBS also exhibited linear agreement with R(2) values greater than 0.9.
conclusionsWith a variety of options, including mass accuracy, merged adducts, and filtering criteria, MultiGlycan-ESI allows automated annotation and quantitation of LC/ESI-MS N-glycan data. The software allows the reliable quantitation of glycan LC/MS data. The software is reliable for automated glycan quantitation, thus facilitating rapid and reliable high-throughput glycomics studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.