ArticleMolecular and cellular biology1989
Hygromycin resistance as a selectable marker in Dictyostelium discoideum.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Analysis of the Microprocessor in Dictyostelium: The Role of RbdB, a dsRNA Binding Protein.PLoS genetics · 2016Article
- Loss of the histidine kinase DhkD results in mobile mounds during development of Dictyostelium discoideum.PloS one · 2013Article
- Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles.Cellular and molecular life sciences : CMLS · 2011Article
- Cell type-specific filamin complex regulation by a novel class of HECT ubiquitin ligase is required for normal cell motility and patterning.Development (Cambridge, England) · 2011Article
- PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis.Developmental cell · 2007Article
- Function of ammonium transporter A in the initiation of culmination of development in Dictyostelium discoideum.Eukaryotic cell · 2006Article
- UGUS, a reporter for use with destabilizing N-termini.Nucleic acids research · 1998Article
- Expression of an activated rasD gene changes cell fate decisions during Dictyostelium development.Molecular biology of the cell · 1997Article
- Dictyostelium discoideum cells lacking the 34,000-dalton actin-binding protein can grow, locomote, and develop, but exhibit defects in regulation of cell structure and movement: a case of partial redundancy.The Journal of cell biology · 1996Article
- Overexpression of myosin motor domains in Dictyostelium: screening of transformants and purification of the affinity tagged protein.Journal of muscle research and cell motility · 1995Article
- The Ca(2+)-binding domains in non-muscle type alpha-actinin: biochemical and genetic analysis.The Journal of cell biology · 1993Article
- A Dictyostelium myosin II lacking a proximal 58-kDa portion of the tail is functional in vitro and in vivo.Molecular biology of the cell · 1992Article
- Shuttle vectors conferring hygromycin B resistance to E. coli and to mammalian cells. Differential expression of carboxyterminal fusion proteins.Molecular biology reports · 1992Article
- Double targeted gene replacement for creating null mutants.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Expression of human antithrombin III in the cellular slime mould Dictyostelium discoideum.Applied microbiology and biotechnology · 1991Article
- Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid.Molecular & general genetics : MGG · 1991Article
- Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo.The Journal of cell biology · 1991Article
- Generation and characterization of Dictyostelium cells deficient in a myosin I heavy chain isoform.The Journal of cell biology · 1990Article
- Molecular complementation of a genetic marker in Dictyostelium using a genomic DNA library.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have constructed an expression cartridge which has the bacterial hygromycin resistance gene (hph) fused to the Dictyostelium discoideum actin 15 promoter, with a segment of 3'-flanking DNA from the actin 15 locus placed downstream of the hph gene to serve as a transcription terminator. The plasmid pDE109, which contained this cartridge and a Dictyostelium origin of replication, transformed D. discoideum with high efficiency under hygromycin selection. The availability of this selectable marker circumvents the previous limitation of having G418 resistance as the only selectable marker for this organism; secondary transformation can now be used to introduce DNA into previously transformed cell lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.