ArticleInsect biochemistry and molecular biology2014
Site-specific, TALENs-mediated transformation of Bombyx mori.
Article in Insect biochemistry and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Gustatory receptors interact with the serotonin pathway to regulate silkmoth stereotyped courtship behavior.BMC biology · 2025Article
- TALEN-mediated homologous-recombination-based fibroin light chain in-fusion expression system inFrontiers in bioengineering and biotechnology · 2024Article
- Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control inInsects · 2023Article
- Engineering a complex, multiple enzyme-mediated synthesis of natural plant pigments in the silkworm,Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genome Editing and Diabetic Cardiomyopathy.Advances in experimental medicine and biology · 2023Article
- Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori.BMC biotechnology · 2021Article
- Silkworm genetic sexing through W chromosome-linked, targeted gene integration.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mass spider silk production through targeted gene replacement inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system.Transgenic research · 2016Article
- Pheromone-Based Pest Management in China: Past, Present, and Future Prospects.Journal of chemical ecology · 2016Review
- CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells.Scientific reports · 2015Article
- Nutritional Control of Insect Reproduction.Current opinion in insect science · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Transposon-based genetic transformation has facilitated insect functional genomics and new strategies of pest management. However, there is a need for alternative, site-specific approaches to overcome limitations of random integration (and associated position-effects) and potential instability of inserted transgenes. Here we describe a transposon-free, site-specific genetic transformation system mediated by transcription activator-like effector nucleases (TALENs) in the silkworm, Bombyx mori, a lepidopteran model insect. We successfully established a site-specific transgenic system with comparable transformation efficiency to transposon-based genetic transformation through microinjection of TALENs mRNA targeting the BmBLOS2 locus and a linearizable donor plasmid encoding an expression cassette of the DsRed2 red fluorescent protein. This system provides a valuable approach for insect transgenesis and will enable future functional gene analysis and generate novel applications in agricultural and medical insect pest-management technologies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.