ArticleMolecules (Basel, Switzerland)2014
Isolation and characterization of a novel lectin from the edible mushroom Stropharia rugosoannulata.
Article in Molecules (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- The Transformation and Protein Expression of the Edible MushroomJournal of fungi (Basel, Switzerland) · 2025Article
- The rise of Stropharia rugosoannulata industry in China: current state and prospects.Applied microbiology and biotechnology · 2025Review
- Nutritional and Therapeutic Potential ofJournal of fungi (Basel, Switzerland) · 2025Review
- Article
- Article
- Integrative Analysis of Transcriptome and Metabolome Sheds Light on Flavonoid Biosynthesis in the Fruiting Body ofJournal of fungi (Basel, Switzerland) · 2024Article
- Nutritional, Bioactive, and Flavor Components of Giant Stropharia (Journal of fungi (Basel, Switzerland) · 2023Review
- Difference in Volatile Aroma Components ofFoods (Basel, Switzerland) · 2023Article
- Whole Genome Sequence of an Edible MushroomJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Widely Targeted Metabolomics Analysis of the Changes to Key Non-volatile Taste Components inFrontiers in nutrition · 2022Article
- Isolation, purification, and structural elucidation ofFrontiers in nutrition · 2022Article
- Macrofungi Cultivation in Shady Forest Areas Significantly Increases Microbiome Diversity, Abundance and Functional Capacity in Soil Furrows.Journal of fungi (Basel, Switzerland) · 2021Article
- Narrative Review: Bioactive Potential of Various Mushrooms as the Treasure of Versatile Therapeutic Natural Product.Journal of fungi (Basel, Switzerland) · 2021Review
- Effects of drying on the structural characteristics and antioxidant activities of polysaccharides fromJournal of food science and technology · 2021Article
- Fejerlectin, a Lectin-like Peptide from the Skin ofACS omega · 2021Article
- Immunomodulatory Effects of Edible and Medicinal Mushrooms and Their Bioactive Immunoregulatory Products.Journal of fungi (Basel, Switzerland) · 2020Review
- Proteins fromPeerJ · 2020Article
- An Inulin-Specific Lectin with Anti-HIV-1 Reverse Transcriptase, Antiproliferative, and Mitogenic Activities from the Edible MushroomBioMed research international · 2019Article
- A New Galactose-Specific Lectin fromIranian journal of biotechnology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, only a few steroids have been isolated from the mushroom Stropharia rugosoannulata which can be cultivated. In this paper, a novel lectin (SRL) with a molecular weight of 38 kDa, and a unique IKSGVYRIVSWQGALGPEAR N-terminal sequence was isolated from S. rugosoannulata, which represents the first protein isolated from the mushroom. The purification methods included (NH4)2SO4 precipitation, ion exchange chromatography on CM-cellulose, Q-Sepharose, and SP-Sepharose, and gel- filtration on Superdex-75. The lectin was adsorbed on all three types of ion exchangers and was purified more than 450-fold. The lectin was stable below 70 °C (with half of the activity preserved at 80 °C), and in the presence of NaOH and HCl solutions up to a concentration of 12.5 mM and 25 mM, respectively. The hemagglutinating activity of SRL was inhibited by inulin. Cd2+ and Hg2+ ions strongly reduced the hemagglutinating activity at concentrations from 1.25 mM to 10 mM. SRL exhibited anti-proliferative activity toward both hepatoma Hep G2 cells and leukemia L1210 cells, with an IC50 of 7 μM and 19 μM, respectively. The activity of HIV-1 reverse transcriptase could also be inhibited by SRL, with an IC50 of 10 μM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.