ArticlePloS one2014
Canonical Wnt signaling activity in early stages of chick lung development.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Pulmonary development in Squamata: Insights from embryonic studies using micro-CT.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Broiler Ascites Syndrome as a Potential Spontaneous Animal Model for Human Pulmonary Arterial Hypertension: A Narrative Review.Life (Basel, Switzerland) · 2026Review
- Retinoic Acid-Mediated Control of Energy Metabolism Is Essential for Lung Branching Morphogenesis.International journal of molecular sciences · 2024Article
- Review
- Review
- Lung branching morphogenesis is accompanied by temporal metabolic changes towards a glycolytic preference.Cell & bioscience · 2021Article
- Noncanonical Wnt planar cell polarity signaling in lung development and disease.Biochemical Society transactions · 2020Review
- It's about time: clocks in the developing lung.The Journal of clinical investigation · 2020Review
- Lysyl hydroxylase 3 increases collagen deposition and promotes pulmonary fibrosis by activating TGFβ1/Smad3 and Wnt/β-catenin pathways.Archives of medical science : AMS · 2020Article
- Differential expression of circRNAs during rat lung development.International journal of molecular medicine · 2019Article
- Retinoic acid regulates avian lung branching through a molecular network.Cellular and molecular life sciences : CMLS · 2017Article
- Canonical Sonic Hedgehog Signaling in Early Lung Development.Journal of developmental biology · 2017Review
- Expression analysis of Shh signaling members in early stages of chick lung development.Histochemistry and cell biology · 2016Article
- Lung branching morphogenesis, in the chicken model, is accompanied by temporal metabolic changes:Porto biomedical journalArticle
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wnt signaling pathway is an essential player during vertebrate embryonic development which has been associated with several developmental processes such as gastrulation, body axis formation and morphogenesis of numerous organs, namely the lung. Wnt proteins act through specific transmembrane receptors, which activate intracellular pathways that regulate cellular processes such as cell proliferation, differentiation and death. Morphogenesis of the fetal lung depends on epithelial-mesenchymal interactions that are governed by several growth and transcription factors that regulate cell proliferation, fate, migration and differentiation. This process is controlled by different signaling pathways such as FGF, Shh and Wnt among others. Wnt signaling is recognized as a key molecular player in mammalian pulmonary development but little is known about its function in avian lung development. The present work characterizes, for the first time, the expression pattern of several Wnt signaling members, such as wnt-1, wnt-2b, wnt-3a, wnt-5a, wnt-7b, wnt-8b, wnt-9a, lrp5, lrp6, sfrp1, dkk1, β-catenin and axin2 at early stages of chick lung development. In general, their expression is similar to their mammalian counterparts. By assessing protein expression levels of active/total β-catenin and phospho-LRP6/LRP6 it is revealed that canonical Wnt signaling is active in this embryonic tissue. In vitro inhibition studies were performed in order to evaluate the function of Wnt signaling pathway in lung branching. Lung explants treated with canonical Wnt signaling inhibitors (FH535 and PK115-584) presented an impairment of secondary branch formation after 48 h of culture along with a decrease in axin2 expression levels. Branching analysis confirmed this inhibition. Wnt-FGF crosstalk assessment revealed that this interaction is preserved in the chick lung. This study demonstrates that Wnt signaling is crucial for precise chick lung branching and further supports the avian lung as a good model for branching studies since it recapitulates early mammalian pulmonary development.
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