ArticleJournal of virology1989
Multiple transcription factor binding sites mediate adenovirus E1A transactivation.
Article in Journal of virology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Integration of a multimeric STAT consensus site into the adenoviral genome to interfere with IFN signaling represents a novel concept to improve oncolytic virotherapy.Journal of virology · 2026Article
- The Inevitable Relationship Between Viruses and RNA Modifications Revealed Through Adenovirus Research.Viruses · 2026Review
- Adeno-associated virus Rep78 protein interacts with protein kinase A and its homolog PRKX and inhibits CREB-dependent transcriptional activation.Journal of virology · 1998Article
- Identification of sequence elements in the human cytomegalovirus DNA polymerase gene promoter required for activation by viral gene products.Journal of virology · 1994Article
- Adenovirus E4orf4 protein reduces phosphorylation of c-Fos and E1A proteins while simultaneously reducing the level of AP-1.Journal of virology · 1992Article
- Upstream box/TATA box order is the major determinant of the direction of transcription.Nucleic acids research · 1991Article
- Conversion of the E1A Cys4 zinc finger to a nonfunctional His2,Cys2 zinc finger by a single point mutation.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- trans-dominant mutants of E1A provide genetic evidence that the zinc finger of the trans-activating domain binds a transcription factor.Molecular and cellular biology · 1991Article
- E1A represses wild-type and F9-selected polyomavirus DNA replication by a mechanism not requiring depression of large tumor antigen transcription.Journal of virology · 1991Article
- Adenovirus E1A protein activates transcription of the E1A gene subsequent to transcription complex formation.Journal of virology · 1991Article
- E1A gene products stimulate in vitro transcription from the adenovirus early region 4 promoter by enhancing a stable preinitiation complex.Virus genes · 1991Article
- E1a-dependent expression of adenovirus genes in OTF963 embryonal carcinoma cells: role of E1a-induced differentiation.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- The human cytomegalovirus 2.7-kilobase RNA promoter contains a functional binding site for the adenovirus major late transcription factor.Journal of virology · 1990Article
- cis-dominant defect in activation of adenovirus type 5 E1b early RNA synthesis.Journal of virology · 1990Article
- The adenovirus EII early promoter has multiple EIA-sensitive elements, two of which function cooperatively in basal and virus-induced transcription.Journal of virology · 1990Article
- Adenovirus early region 3 promoter regulation by E1A/E1B is independent of alterations in DNA binding and gene activation of CREB/ATF and AP1.Journal of virology · 1990Article
- Two distinct cellular proteins interact with the EIa-responsive element of an adenovirus early promoter.Journal of virology · 1990Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We studied the response of simple synthetic promoter regions to transactivation by the adenovirus early region 1A (E1A) protein. Binding sites for one or two host cell transcription factors were substituted for the E1B promoter region in reconstructed virus mutants, and the response to E1A transactivation was assayed during the early phase of infection. We found that a single CREB/ATF binding site resulted in a surprisingly strong promoter which responded to E1A. A CREB/ATF binding site placed upstream of the E1B TATA box behaved much like the wild-type E1B promoter, which is composed of a single Sp1 binding site plus a TATA box. A single E2F binding site resulted in an extremely weak promoter which did not respond to E1A, much like a single Sp1 site. Two E2F sites in an inverted orientation with the same spacing as in the adenovirus type 2 E2 early promoter produced a strong, E1A-responsive promoter. Substitution of the E4 TATA box region for the E1B TATA box region produced a promoter about five times stronger than the wild-type E1B promoter in the absence of E1A. However, the E4 TATA box substitution did not respond significantly to E1A transactivation. These results directly demonstrate that many different transcription factor binding sites, including the E1B TATA box, a CREB/ATF binding site, and two E2F sites, can mediate E1A transactivation. Other transcription factor binding sites cannot mediate an E1A response; these other sites include the E4 TATA box, a single Sp1 binding site, and a single E2F binding site. Implications of these findings for the mechanism of E1A transactivation are discussed.
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