Evidence map›Paper›PMID 25457275›Full record

ArticleCytotherapy2015

Clinical mesenchymal stromal cell products undergo functional changes in response to freezing.

Kathryn Pollock, Darin Sumstad, Diane Kadidlo, David H McKenna, Allison Hubel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  9. The Evolving Landscape of Potency Assays.Advances in experimental medicine and biology · 2023
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  20. The role of preservation in the variability of regenerative medicine products.Regenerative engineering and translational medicine · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kathryn PollockDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Darin SumstadMolecular and Cellular Therapy Center, University of Minnesota, Minneapolis, Minnesota, USA.
Diane KadidloMolecular and Cellular Therapy Center, University of Minnesota, Minneapolis, Minnesota, USA.
David H McKennaMolecular and Cellular Therapy Center, University of Minnesota, Minneapolis, Minnesota, USA.
Allison HubelDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, Minnesota, USA. Electronic address: hubel001@umn.edu.

Funding

Integrated training in development and clinical practice of cell-based therapiesR25HL128372 · NHLBI · UNIVERSITY OF MINNESOTA · PI HUBEL, ALLISON, MCKENNA, DAVID HURLEY · 2015 to 2019
$553k
Technology platform for development of multi-component preservation solutionR21EB016247 · NIBIB · UNIVERSITY OF MINNESOTA · PI HUBEL, ALLISON · 2013 to 2014
$387k
NHLBI NIH HHS R25 HL128372NIBIB NIH HHS R21 EB016247NIBIB NIH HHS R21EB016247PHS HHS HHSN268201000008C
6 · The paper itself

Abstract

BACKGROUND

aimsCurrent methods of mesenchymal stromal cell (MSC) cryopreservation result in variable post-thaw recovery and phenotypic changes caused by freezing. The objective of this investigation was to determine the influence of ex vivo cell expansion on phenotype of MSCs and the response of resulting phenotypes to freezing and thawing.

methodsHuman bone marrow aspirate was used. MSCs were isolated and cells were assessed for total count, viability, apoptosis and senescence over 6 passages (8-10 doublings/passage) in ex vivo culture. One half of cells harvested at each passage were re-plated for continued culture and the other half were frozen at 1°C/min in a controlled-rate freezer. Frozen samples were stored in liquid nitrogen, thawed and reassessed for total cell count, viability and senescence immediately and 48 h after thaw.

resultsViability did not differ significantly between samples before freeze or after thaw. Senescence increased over time in pre-freeze culture and was significantly higher in one sample that had growth arrest both before freeze and after thaw. Freezing resulted in similar initial post-thaw recovery in all samples, but 48-h post-thaw growth arrest was observed in the sample with high senescence only.

conclusionsHigh pre-freeze senescence appears to correlate with poor post-thaw function in MSC samples, but additional studies are necessary to obtain a sample sizes large enough to quantify results.

Indexed as

CryopreservationAdultApoptosisCell CountCell CycleCell ProliferationCell SurvivalFemaleFreezingHumansMaleMesenchymal Stem Cellsapoptosisfreezingfunctionmesenchymal stromal cellssenescenceviability

Identifiers

PMID25457275
PMCPMC4274232

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.