ArticleJournal of virological methods2015
Rapid detection and differentiation of dengue virus serotypes by NS1 specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay in patients presenting to a tertiary care hospital in Hyderabad, India.
Article in Journal of virological methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Genomic diversity analysis enables development of pan-Dengue Toehold RNA sensors.PLoS neglected tropical diseases · 2026Article
- A Novel Loop-Mediated Isothermal Amplification (LAMP) Assay for Detecting Salmonella Ser. Typhimurium in Egg Products.Journal of AOAC International · 2025Article
- Development of LAMP assay for early detection ofPeerJ · 2025Article
- Challenges in Direct Detection of Flaviviruses: A Review.Pathogens (Basel, Switzerland) · 2023Review
- Association of Dengue Virus Serotypes 1&2 with Severe Dengue Having Deletions in Their 3'Untranslated Regions (3'UTRs).Microorganisms · 2023Article
- Analytical and diagnostic performance characteristics of reverse-transcriptase loop-mediated isothermal amplification assays for dengue virus serotypes 1-4: A scoping review to inform potential use in portable molecular diagnostic devices.PLOS global public health · 2023Article
- Detection of dengue virus serotypes by single-tube multiplex RT-PCR and multiplex real-time PCR assay.Medical journal, Armed Forces India · 2022Article
- Development of a Singleplex Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Quantification.Viruses · 2022Article
- Evidence of dengue virus in eviscerated specimens of panophthalmitis secondary to dengue fever: A possible cause-effect phenomenon.Indian journal of ophthalmology · 2022Article
- Electropolymerized-molecularly imprinted polymers (E-MIPS) as sensing elements for the detection of dengue infection.Analytical and bioanalytical chemistry · 2022Article
- Taguchi array optimization of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for sensitive and rapid detection of dengue virus serotype 2.Biotechnology letters · 2021Article
- Detecting wMel Wolbachia in field-collected Aedes aegypti mosquitoes using loop-mediated isothermal amplification (LAMP).Parasites & vectors · 2019Article
- A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos.PloS one · 2019Article
- Potential Point-of-Care Testing for Dengue Virus in the Field.Journal of clinical microbiology · 2018Article
- Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype.PLoS neglected tropical diseases · 2018Article
- Evaluation of fluorimetry and direct visualization to interpret results of a loop-mediated isothermal amplification kit to detect Leishmania DNA.Parasites & vectors · 2018Article
- Progress and Challenges towards Point-of-Care Diagnostic Development for Dengue.Journal of clinical microbiology · 2017Review
- Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay.Frontiers in plant science · 2016Article
- Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1-4.BMC microbiology · 2015Article
- Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus.Diagnostic microbiology and infectious disease · 2015Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early and rapid detection of dengue virus (DENV) infection during the acute phase of illness is crucial for proper patient management and prevention of the spread of the infection. In the present study, the standardization and validation of a one step, four tube reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for rapid detection and serotyping of the DENV targeting NS1 gene using the Genie® II flourometer was carried out. The performance of the RT-LAMP was compared to RT-PCR, CDC 1-4 Real time PCR and the NS1 antigen ELISA, IgM and IgG anti DENV antibodies. Acute DENV infection was confirmed in 250/300 patients suspected clinically of DENV infection. RT- LAMP and CDC 1-4 Real time PCR assay was positive in 148/250 patients, while 92/250 patients were positive for anti- Dengue IgM and IgG antibodies. The RT-LAMP assay and the CDC real-time RT-PCR assay showed high concordance (k=1.0). The detection rate of acute DENV infection improved to 96% (240/250) when the results of RT-LAMP were combined with NS1 Ag, IgM and IgG ELISA. The RT-LAMP had a detection limit of 100 copies for DEN-1 and DEN-2, 10 copies for DEN-3 and DEN-4 compared to 1000 copies for DEN-1 and DEN-2, 100 copies for DEN-3 and DEN-4 by the conventional RT-PCR. The assay showed 100% specificity. The RT-LAMP assay developed in this study has potential use for early clinical diagnosis, serotyping and surveillance of DENV infection in endemic countries such as India.
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