Evidence map›Paper›PMID 25455074›Full record

ArticleStem cell reports2014

RB maintains quiescence and prevents premature senescence through upregulation of DNMT1 in mesenchymal stromal cells.

Shih-Pei Lin, Fang-Yao Chiu, Yu Wang, Men-Luh Yen, Shou-Yen Kao, Shih-Chieh Hung

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 53 citations in OpenAlex.

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  14. Frontiers in cell and developmental biology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Shih-Pei LinInstitute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan, ROC.
Fang-Yao ChiuDepartment of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan, ROC.
Yu WangDepartment of Dentistry Sciences, National Yang-Ming University, Taipei 112, Taiwan, ROC; Department of Stomatology, Taipei Veterans General Hospital, Taipei 112, Taiwan, ROC.
Men-Luh YenDepartments of Primary Care Medicine and Obstetrics/Gynecology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei 100, Taiwan, ROC.
Shou-Yen KaoDepartment of Dentistry Sciences, National Yang-Ming University, Taipei 112, Taiwan, ROC; Department of Stomatology, Taipei Veterans General Hospital, Taipei 112, Taiwan, ROC. Electronic address: sykao@vghtpe.gov.tw.
Shih-Chieh HungInstitute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan, ROC; Institute of Pharmacology, National Yang-Ming University, Taipei 112, Taiwan, ROC; Institute of Traditional Medicine, Faculty of Medicine, National Yang-Ming University, Taipei 112, Taiwan, ROC; Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan, ROC; Stem Cell Laboratory, Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 112, Taiwan, ROC; Institute of Biomedical Sciences, Academia Sinica, Taipei 105, Taiwan, ROC. Electronic address: hungsc@vghtpe.gov.tw.
National Yang Ming Chiao Tung University · TWNational Taiwan University Hospital · TWTaipei Veterans General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many cell therapies currently being tested are based on mesenchymal stromal cells (MSCs). However, MSCs start to enter the senescent state upon long-term expansion. The role of retinoblastoma (RB) protein in regulating MSC properties is not well studied. Here, we show that RB levels are higher in early-passage MSCs compared with late-passage MSCs. RB knockdown induces premature senescence and reduced differentiation potentials in early-passage MSCs. RB overexpression inhibits senescence and increases differentiation potentials in late-passage MSCs. Expression of DNMT1, but not DNMT3A or DNMT3B, is also higher in early-passage MSCs than in late-passage MSCs. Furthermore, DNMT1 knockdown in early-passage MSCs induces senescence and reduces differentiation potentials, whereas DNMT1 overexpression in late-passage MSCs has the opposite effect. These results demonstrate that RB expressed in early-passage MSCs upregulates DNMT1 expression and inhibits senescence in MSCs. Therefore, genetic modification of RB could be a way to improve the efficiency of MSCs in clinical use.

Indexed as

Gene Expression RegulationAnimalsBone Marrow TransplantationCell DifferentiationCell ProliferationCellular SenescenceDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesGene ExpressionGene Knockdown TechniquesHumansMesenchymal Stem CellsMesenchymal Stem Cell TransplantationMiceProtein BindingProto-Oncogene Proteins c-junDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNMT1 protein, humanDnmt1 protein, mouseProto-Oncogene Proteins c-junRetinoblastoma Protein

Identifiers

PMID25455074
PMCPMC4264040
OpenAlexW2108593601

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.