ArticleTicks and tick-borne diseases2015
Amblyomma americanum tick calreticulin binds C1q but does not inhibit activation of the classical complement cascade.
Article in Ticks and tick-borne diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- One Health tick bite exposure biomarkers against Ixodes ricinus salivary peptides.Research square · 2026Article
- Antibodies to Tick Salivary Antigens AV422 and Calreticulin in Patients With Spotted Fever Group Rickettsioses and Other Febrile Illnesses in Northern Tanzania.The Journal of infectious diseases · 2026Article
- Binding Molecules in Tick Saliva for Targeting Host Cytokines, Chemokines, and Beyond.Biomolecules · 2024Review
- RecombinantPathogens (Basel, Switzerland) · 2024Article
- The many faces of parasite calreticulin.Frontiers in immunology · 2023Review
- Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer.Scientific reports · 2022Article
- Protein profiling of hemolymph in Haemaphysalis flava ticks.Parasites & vectors · 2022Article
- Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions.Frontiers in cellular and infection microbiology · 2022Review
- Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs.BMC genomics · 2021Article
- Identification ofPathogens (Basel, Switzerland) · 2020Article
- The Interactions of Parasite Calreticulin With Initial Complement Components: Consequences in Immunity and Virulence.Frontiers in immunology · 2020Review
- TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2019Article
- A bite so sweet: the glycobiology interface of tick-host-pathogen interactions.Parasites & vectors · 2018Review
- Disruption of blood meal-responsive serpins prevents Ixodes scapularis from feeding to repletion.Ticks and tick-borne diseases · 2018Article
- The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission.Frontiers in cellular and infection microbiology · 2017Review
- Immunomodulatory Effects ofFrontiers in cellular and infection microbiology · 2017Article
- DecipheringFrontiers in cellular and infection microbiology · 2017Review
- Saliva of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) inhibits classical and alternative complement pathways.Parasites & vectors · 2016Article
- Target validation of highly conserved Amblyomma americanum tick saliva serine protease inhibitor 19.Ticks and tick-borne diseases · 2016Article
- Ixodes scapularis Tick Saliva Proteins Sequentially Secreted Every 24 h during Blood Feeding.PLoS neglected tropical diseases · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In this study we characterized Amblyomma americanum (Aam) tick calreticulin (CRT) homolog in tick feeding physiology. In nature, different tick species can be found feeding on the same animal host. This suggests that different tick species found feeding on the same host can modulate the same host anti-tick defense pathways to successfully feed. From this perspective it's plausible that different tick species can utilize universally conserved proteins such as CRT to regulate and facilitate feeding. CRT is a multi-functional protein found in most taxa that is injected into the vertebrate host during tick feeding. Apart from it's current use as a biomarker for human tick bites, role(s) of this protein in tick feeding physiology have not been elucidated. Here we show that annotated functional CRT amino acid motifs are well conserved in tick CRT. However our data show that despite high amino acid identity levels to functionally characterized CRT homologs in other organisms, AamCRT is apparently functionally different. Pichia pastoris expressed recombinant (r) AamCRT bound C1q, the first component of the classical complement system, but it did not inhibit activation of this pathway. This contrast with reports of other parasite CRT that inhibited activation of the classical complement pathway through sequestration of C1q. Furthermore rAamCRT did not bind factor Xa in contrast to reports of parasite CRT binding factor Xa, an important protease in the blood clotting system. Consistent with this observation, rAamCRT did not affect plasma clotting or platelet aggregation. We discuss our findings in the context of tick feeding physiology.
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