ArticleProceedings of the National Academy of Sciences of the United States of America2014
Label-free imaging of Schwann cell myelination by third harmonic generation microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Simultaneous three-photon and optical coherence microscopy deep within an intact mouse brain.Npj imaging · 2026Article
- Label-free tracking of subcortical white matter degradationBiomedical optics express · 2026Article
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.Communications biology · 2025Article
- Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration.Biophysical reviews · 2024Review
- Discontinuity third harmonic generation microscopy for label-free imaging and quantification of intraepidermal nerve fibers.Cell reports methods · 2024Article
- Live Mapping of the Brain Extracellular Matrix and Remodeling in Neurological Disorders.Small methods · 2024Article
- Third harmonic imaging contrast from tubular structures in the presence of index discontinuity.Scientific reports · 2023Article
- H-ABC tubulinopathy revealed by label-free second harmonic generation microscopy.Scientific reports · 2022Article
- Multiphoton microscopy for label-free multicolor imaging of peripheral nerve.Journal of biomedical optics · 2022Article
- Advances in nonlinear optical microscopy techniques for in vivo and in vitro neuroimaging.Biophysical reviews · 2021Review
- A near-infrared AIE fluorescent probe for myelin imaging: From sciatic nerve to the optically cleared brain tissue in 3D.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Schwann cell interactions during the development of the peripheral nervous system.Developmental neurobiology · 2021Review
- High-pulse-energy multiphoton imaging of neurons and oligodendrocytes in deep murine brain with a fiber laser.Scientific reports · 2021Article
- Label-Free Multiphoton Microscopy: Much More Than Fancy Images.International journal of molecular sciences · 2021Review
- Quantitative birefringence microscopy for imaging the structural integrity of CNS myelin following circumscribed cortical injury in the rhesus monkey.Neurophotonics · 2021Article
- Quantitative third-harmonic generation imaging of mouse visual cortex areas reveals correlations between functional maps and structural substrates.Biomedical optics express · 2020Article
- Slide-free clinical imaging of melanin with absolute quantities using label-free third-harmonic-generation enhancement-ratio microscopy.Biomedical optics express · 2020Article
- Review
- Quantitative Third Harmonic Generation Microscopy for Assessment of Glioma in Human Brain Tissue.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
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6 authors.
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Abstract
Understanding the dynamic axon-glial cell interaction underlying myelination is hampered by the lack of suitable imaging techniques. Here we demonstrate third harmonic generation microscopy (THGM) for label-free imaging of myelinating Schwann cells in live culture and ex vivo and in vivo tissue. A 3D structure was acquired for a variety of compact and noncompact myelin domains, including juxtaparanodes, Schmidt-Lanterman incisures, and Cajal bands. Other subcellular features of Schwann cells that escape traditional optical microscopies were also visualized. We tested THGM for morphometry of compact myelin. Unlike current methods based on electron microscopy, g-ratio could be determined along an extended length of myelinated fiber in the physiological condition. The precision of THGM-based g-ratio estimation was corroborated in mouse models of hypomyelination. Finally, we demonstrated the feasibility of THGM to monitor morphological changes of myelin during postnatal development and degeneration. The outstanding capabilities of THGM may be useful for elucidation of the mechanism of myelin formation and pathogenesis.
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