ArticleProceedings of the National Academy of Sciences of the United States of America2014
Power transduction of actin filaments ratcheting in vitro against a load.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Anisotropic Cellular Forces Drive Hexagonal-to-Tetragonal Tiling Transitions in the Drosophila Eye.Development, growth & differentiation · 2026Article
- Nascent adhesions shorten the period of lamellipodium protrusion through the Brownian ratchet mechanism.Molecular biology of the cell · 2023Article
- Combined Traction Force-Atomic Force Microscopy Measurements of Neuronal Cells.Biomimetics (Basel, Switzerland) · 2022Article
- Force-velocity relation and load-sharing in the linear polymerization ratchet revisited: the effects of barrier diffusion.The European physical journal. E, Soft matter · 2022Article
- Mouse T cell priming is enhanced by maturation-dependent stiffening of the dendritic cell cortex.eLife · 2020Article
- Lamellipodium tip actin barbed ends serve as a force sensor.Genes to cells : devoted to molecular & cellular mechanisms · 2019Article
- Polymerisation force of a rigid filament bundle: diffusive interaction leads to sublinear force-number scaling.Scientific reports · 2018Article
- Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction.F1000Research · 2018Review
- Article
- Amplification of actin polymerization forces.The Journal of cell biology · 2016Article
- Emergence of an Apical Epithelial Cell Surface In Vivo.Developmental cell · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin cytoskeleton has the unique capability of producing pushing forces at the leading edge of motile cells without the implication of molecular motors. This phenomenon has been extensively studied theoretically, and molecular models, including the widely known Brownian ratchet, have been proposed. However, supporting experimental work is lacking, due in part to hardly accessible molecular length scales. We designed an experiment to directly probe the mechanism of force generation in a setup where a population of actin filaments grows against a load applied by magnetic microparticles. The filaments, arranged in stiff bundles by fascin, are constrained to point toward the applied load. In this protrusion-like geometry, we are able to directly measure the velocity of filament elongation and its dependence on force. Using numerical simulations, we provide evidence that our experimental data are consistent with a Brownian ratchet-based model. We further demonstrate the existence of a force regime far below stalling where the mechanical power transduced by the ratcheting filaments to the load is maximal. The actin machinery in migrating cells may tune the number of filaments at the leading edge to work in this force regime.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.