ArticlePlant physiology2015
Iron-binding E3 ligase mediates iron response in plants by targeting basic helix-loop-helix transcription factors.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
136 citing papers in PubMed, 1 synthesis or guideline pooled it, 297 citations in OpenAlex.
- Meta-Analysis of Iron Excess Stress in Rice: Genes and Mechanisms of Tolerance to Acidic Soil.Physiologia plantarumPooled it
- MPK3-mediated phosphorylation inactivates MYB4 to promote FIT-dependent iron acquisition.EMBO reports · 2026Article
- Apoplast Engineering Unveiled: Multi-Target Strategies to Disarm Pathogens and Elevate Global Food Security.Plant, cell & environment · 2026Review
- Accelerating iron biofortification in millets: progress, challenges, and future prospects.Planta · 2026Review
- CRISPR/Cas9 Editing of the Wheat Iron Sensor TaHRZ1 Confirms Its Conserved Role in Iron Homeostasis and Allocation in Grains.Plant, cell & environment · 2026Article
- Whole genome sequencing-based multi-locus association mapping for kernel iron, zinc and protein content in groundnut.Scientific reports · 2026Article
- Identification of a novel gene inAntimicrobial agents and chemotherapy · 2026Article
- Establishment of a Non-transgenic Iron-Biofortified Rice Line Using a Novel HRZ1 Mutation.Rice (New York, N.Y.) · 2026Article
- Identification and characterization of U-box E3 ubiquitin ligase genes (ChPUBs) in Cardamine hupingshanensis under abiotic stress.BMC genomics · 2026Article
- Hit me with your best shot: combining gene editing events to deliver iron and zinc biofortified rice.Frontiers in plant science · 2026Review
- Integrating microbial siderophores into concepts of plant iron nutrition.Nature plants · 2026Review
- A 'Candidatus Liberibacter asiaticus' effector SDE2470 facilitates citrus transcription factor CsVOZ2 degradation via BRUTUS E3 ligases.PLoS pathogens · 2025Article
- Age-dependent differential iron deficiency responses of rosette leaves during reproductive stages in Arabidopsis thaliana.Journal of experimental botany · 2025Article
- Pumping iron, keeping FIT: How MYB30 regulates FIT stability during plant iron deficiency.The Plant cell · 2025Article
- The transcription factor MYB30 promotes iron homeostasis by maintaining the stability of the FIT transcription factor.The Plant cell · 2025Article
- Metal-sensing properties of the disordered loop from the Arabidopsis metal transceptor IRT1.The Biochemical journal · 2025Article
- An RNA helicase coordinates with iron signal regulators to alleviate chilling stress in Arabidopsis.Nature communications · 2025Article
- Iron-sensing and redox properties of the hemerythrin-like domains of Arabidopsis BRUTUS and BRUTUS-LIKE2 proteins.Nature communications · 2025Article
- BRUTUS links iron with legume-rhizobia symbiosis.Nature plants · 2025Article
- The BRUTUS iron sensor and E3 ligase facilitates soybean root nodulation by monoubiquitination of NSP1.Nature plants · 2025Article
76 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iron uptake and metabolism are tightly regulated in both plants and animals. In Arabidopsis (Arabidopsis thaliana), BRUTUS (BTS), which contains three hemerythrin (HHE) domains and a Really Interesting New Gene (RING) domain, interacts with basic helix-loop-helix transcription factors that are capable of forming heterodimers with POPEYE (PYE), a positive regulator of the iron deficiency response. BTS has been shown to have E3 ligase capacity and to play a role in root growth, rhizosphere acidification, and iron reductase activity in response to iron deprivation. To further characterize the function of this protein, we examined the expression pattern of recombinant ProBTS::β-GLUCURONIDASE and found that it is expressed in developing embryos and other reproductive tissues, corresponding with its apparent role in reproductive growth and development. Our findings also indicate that the interactions between BTS and PYE-like (PYEL) basic helix-loop-helix transcription factors occur within the nucleus and are dependent on the presence of the RING domain. We provide evidence that BTS facilitates 26S proteasome-mediated degradation of PYEL proteins in the absence of iron. We also determined that, upon binding iron at the HHE domains, BTS is destabilized and that this destabilization relies on specific residues within the HHE domains. This study reveals an important and unique mechanism for plant iron homeostasis whereby an E3 ubiquitin ligase may posttranslationally control components of the transcriptional regulatory network involved in the iron deficiency response.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.