ArticleAnnals of botany2015
Immunolabelling of intervessel pits for polysaccharides and lignin helps in understanding their hydraulic properties in Populus tremula × alba.
Article in Annals of botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 53 citations in OpenAlex.
- Seasonal variation of vessel pits in sapwood: microscopical analyses of the morphology and chemical components of pit membrane encrustations in Fraxinus mandschurica.Annals of botany · 2024Article
- Exploring Lignification Complexity in Plant Cell Walls with Airyscan Super-resolution Microscopy and Bioorthogonal Chemistry.Chemical & biomedical imaging · 2023Article
- Structural variation and spatial polysaccharide profiling of intervessel pit membranes in grapevine.Annals of botany · 2022Article
- Investigating Effects of Bordered Pit Membrane Morphology and Properties on Plant Xylem Hydraulic Functions-A Case Study from 3D Reconstruction and Microflow Modelling of Pit Membranes in Angiosperm Xylem.Plants (Basel, Switzerland) · 2020Review
- The Spatio-Temporal Distribution of Cell Wall-Associated Glycoproteins During Wood Formation inFrontiers in plant science · 2020Article
- Comparative analysis of remodelling of the plant-microbe interface in Pisum sativum and Medicago truncatula symbiotic nodules.Protoplasma · 2019Article
- Visualizing Embolism Propagation in Gas-Injected Leaves.Plant physiology · 2019Article
- Infrared Nanospectroscopy Reveals the Chemical Nature of Pit Membranes in Water-Conducting Cells of the Plant Xylem.Plant physiology · 2018Article
- Dynamic Changes of Pectin Epitopes in Cell Walls during the Development of the Procambium-Cambium Continuum in Poplar.International journal of molecular sciences · 2017Article
- Article
- Immunogold scanning electron microscopy can reveal the polysaccharide architecture of xylem cell walls.Journal of experimental botany · 2017Article
- Xylem Vessel Diameter Affects the Compartmentalization of the Vascular PathogenFrontiers in plant science · 2017Article
- The Parenchyma of Secondary Xylem and Its Critical Role in Tree Defense against Fungal Decay in Relation to the CODIT Model.Frontiers in plant science · 2016Review
- The chemical identity of intervessel pit membranes in Acer challenges hydrogel control of xylem hydraulic conductivity.AoB PLANTS · 2016Article
- The cell biology of lignification in higher plants.Annals of botany · 2015Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsThe efficiency and safety functions of xylem hydraulics are strongly dependent on the pits that connect the xylem vessels. However, little is known about their biochemical composition and thus about their hydraulic properties. In this study, the distribution of the epitopes of different wall components (cellulose, hemicelluloses, pectins and lignins) was analysed in intervessel pits of hybrid poplar (Populus tremula × alba).
methodsImmunogold labelling with transmission electron microscopy was carried out with a set of antibodies raised against different epitopes for each wall polysaccharide type and for lignins. Analyses were performed on both immature and mature vessels. The effect of sap ionic strength on xylem conductance was also tested. KEY
resultsIn mature vessels, the pit membrane (PM) was composed of crystalline cellulose and lignins. None of the hemicellulose epitopes were found in the PM. Pectin epitopes in mature vessels were highly concentrated in the annulus, a restricted area of the PM, whereas they were initially found in the whole PM in immature vessels. The pit border also showed a specific labelling pattern, with higher cellulose labelling compared with the secondary wall of the vessel. Ion-mediated variation of 24 % was found for hydraulic conductance.
conclusionsCellulose microfibrils, lignins and annulus-restricted pectins have different physicochemical properties (rigidity, hydrophobicity, porosity) that have different effects on the hydraulic functions of the PM, and these influence both the hydraulic efficiency and vulnerability to cavitation of the pits, including ion-mediated control of hydraulic conductance. Impregnation of the cellulose microfibrils of the PM with lignins, which have low wettability, may result in lower cavitation pressure for a given pore size and thus help to explain the vulnerability of this species to cavitation.
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